Screening Antibacterial Agent from Crude Extract of Marine-Derived Fungi Associated with Soft Corals against MDR-Staphylococcus haemolyticus

2nd International Conference on Tropical and Coastal Region Eco Development 2016

地球科学;生态环境科学

Sabdaningsih, A.^1 ; Cristianawati, O.^1 ; Sibero, M.T.^1 ; Nuryadi, H.^2 ; Radjasa, O.K.^3,4 ; Sabdono, A.^4 ; Trianto, A.^4

Coastal Resources Management, Faculty of Fisheries and Marine Sciences, Diponegoro University, Tembalang, Semarang

50275, Indonesia^1

Tropical Marine Biotechnology Laboratory, Central Laboratory of Research and Services, Diponegoro University, Tembalang, Semarang

50275, Indonesia^2

Ministry of Research, Technology and Higher Education of the Republic of Indonesia, 10270, Indonesia^3

Department of Marine Sciences, Faculty of Fisheries and Marine Sciences, Diponegoro University, Tembalang, Semarang

50275, Indonesia^4

关键词: antibacterial;    Multidrug resistants;    Nosocomial pathogens;    Soft corals;    Staphylococcus haemolyticus;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/55/1/012026/pdf
DOI  :  10.1088/1755-1315/55/1/012026

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

Multidrug resistant Staphylococcus haemolyticus is a Gram-positive bacteria and member of coagulase negative staphylococci (CoNS) which has the highest level of antimicrobial resistance. This nosocomial pathogen due to skin or soft tissue infections, bacteremia, septicemia, peritonitis, otitis media, meningitis and urinary tract infections. The ability to produce enterotoxins, hemolysins, biofilm, and cytotoxins could be an important characteristic for the successful of infection. Marine-derived fungi have potency as a continuous supply of bioactive compound. The aim of this research was screening antibacterial agent from crude extracts of marine-derived fungi associated with soft corals against MDR-S. haemolyticus. Among 23 isolates of marine-derived fungi isolated from 7 soft corals, there were 4 isolates active against MDR-S. haemolyticus. The screening was conducted by using agar plug diffusion method. Isolate PPSC-27-A4 had the highest antibacterial activity with diameter 23±9,6 mm. The crude extract from this isolate had been confirmed to antibacterial susceptibility test and it had the highest antibacterial activity in 12.2 mm with concentration of 300μg/ml from mycelia extract. PPSC-27-A4 had been characterized in molecular, based on the sequence analysis of 18S rRNA, PPSC-27-A4 isolate was identified as Trichoderma longibrachiatum.