Antibacterial Properties of Mussel-Inspired Polydopamine Coatings Prepared by a Simple Two-Step Shaking-Assisted Method

Frontiers in Chemistry

Kevin Thomson¹  Md. Saleh Akram Bhuiyan¹  Rattapol Pinnaratip¹  Elizabeth Polega¹  Bruce P. Lee¹  Pegah Kord Forooshani¹  Lei Pan²  Yuesheng Gao²  Kathryn A. Perrine³  Mikhail Trought³  Chito Kendrick⁴ 

[1] Department of Biomedical Engineering, Michigan Technological University, Houghton, MI, United States;Department of Chemical Engineering, Michigan Technological University, Houghton, MI, United States;Department of Chemistry, Michigan Technological University, Houghton, MI, United States;Department of Electrical Engineering, Michigan Technological University, Houghton, MI, United States;

关键词: catechol;    polydopamine;    coating;    hydrogen peroxide;    antibacterial;    surgical mesh;   

DOI  :  10.3389/fchem.2019.00631

来源: DOAJ

【 摘 要 】

A simple two-step, shaking-assisted polydopamine (PDA) coating technique was used to impart polypropylene (PP) mesh with antimicrobial properties. In this modified method, a relatively large concentration of dopamine (20 mg ml−1) was first used to create a stable PDA primer layer, while the second step utilized a significantly lower concentration of dopamine (2 mg ml−1) to promote the formation and deposition of large aggregates of PDA nanoparticles. Gentle shaking (70 rpm) was employed to increase the deposition of PDA nanoparticle aggregates and the formation of a thicker PDA coating with nano-scaled surface roughness (RMS = 110 nm and Ra = 82 nm). Cyclic voltammetry experiment confirmed that the PDA coating remained redox active, despite extensive oxidative cross-linking. When the PDA-coated mesh was hydrated in phosphate saline buffer (pH 7.4), it was activated to generate 200 μM hydrogen peroxide (H2O2) for over 48 h. The sustained release of low doses of H2O2 was antibacterial against both gram-positive (Staphylococcus epidermidis) and gram-negative (Escherichia coli) bacteria. PDA coating achieved 100% reduction (LRV ~3.15) when incubated against E. coli and 98.9% reduction (LRV ~1.97) against S. epi in 24 h.

【 授权许可】

Unknown