Altered gut microbiome profile in patients with knee osteoarthritis

Frontiers in Microbiology

Microbiology

Yifan Wu¹  Yi Gong¹  Yanli Liu¹  Ruitian Huang¹  Feihong Chen¹  Chaowei Wang¹  Xi Wang²  Hongmou Zhao³  Sijie Chen⁴  Shujin Li⁴  Minhan Hu⁴  Feiyu Zhang⁴  Yujie Ning⁴  Xiong Guo⁵ 

[1] Department of Occupational and Environmental Health, School of Public Health, Xi'an Jiaotong University Health Science Center, Xi’an, Shaanxi, China;Department of Occupational and Environmental Health, School of Public Health, Xi'an Jiaotong University Health Science Center, Xi’an, Shaanxi, China;Key Laboratory of Trace Elements and Endemic Diseases, School of Public Health, Xi’an Jiaotong University Health Science Center, National Health and Family Planning Commission, Xi’an, Shaanxi, China;Foot and Ankle Surgery Department, Honghui Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China;Key Laboratory of Trace Elements and Endemic Diseases, School of Public Health, Xi’an Jiaotong University Health Science Center, National Health and Family Planning Commission, Xi’an, Shaanxi, China;Key Laboratory of Trace Elements and Endemic Diseases, School of Public Health, Xi’an Jiaotong University Health Science Center, National Health and Family Planning Commission, Xi’an, Shaanxi, China;Clinical Research Center for Endemic Disease of Shaanxi Province, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China;

关键词: osteoarthritis;    16s rDNA sequencing;    metagenomic sequencing;    cartilage-gut-microbiome axis;    gut microbiota biomarkers;   

DOI  :  10.3389/fmicb.2023.1153424

 received in 2023-02-03, accepted in 2023-04-26,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionOsteoarthritis (OA) is a kind of chronic, degenerative disorder with unknown causes. In this study, we aimed to improve our understanding of the gut microbiota profile in patients with knee OA.Methods16S rDNA gene sequencing was performed to detect the gut microbiota in fecal samples collected from the patients with OA (n = 32) and normal control (NC, n = 57). Then the metagenomic sequencing was used to identify the genes or functions linked with gut microbial changes at the species level in the fecal samples from patients with OA and NC groups.ResultsThe Proteobacteria was identified as dominant bacteria in OA group. We identified 81 genera resulted significantly different in abundance between OA and NC. The abundance of Agathobacter, Ruminococcus, Roseburia, Subdoligranulum, and Lactobacillus showed significant decrease in the OA compared to the NC. The abundance of genera Prevotella_7, Clostridium, Flavonifractor and Klebsiella were increasing in the OA group, and the families Lactobacillaceae, Christensenellaceae, Clostridiaceae_1 and Acidaminococcaceae were increasing in the NC. The metagenomic sequencing showed that the abundance of Bacteroides stercoris, Bacteroides vulgatus and Bacteroides uniformis at the species level were significantly decreasing in the OA, and the abundance of Escherichia coli, Klebsiella pneumoniae, Shigella flexneri and Streptococcus salivarius were significantly increased in OA.DiscussionThe results of our study interpret a comprehensive profile of the gut microbiota in patients with knee OA and offer the evidence that the cartilage-gut-microbiome axis could play a crucial role in underlying the mechanisms and pathogenesis of OA.

【 授权许可】

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Copyright © 2023 Wang, Wu, Liu, Chen, Chen, Zhang, Li, Wang, Gong, Huang, Hu, Ning, Zhao and Guo.