Significant difference of the culturable anaerobic microbial species in GujingTribute pitmud with different ages

2018 5th International Conference on Advanced Composite Materials and Manufacturing Engineering

Li, Junwei^1 ; He, Hongkui^2 ; Cao, Runjie^2 ; Li, Anjun^2 ; Zhang, Zhizhou^1,3

School of Marine Science and Technology, Marine Anti-fouling Engineering Technology Center of Shandong Province, Harbin Institute of Technology, Harbin

150006, China^1

Anhui GuJingTribute Liquor Ltd, Bozhou, Anhui

236800, China^2

Shandong Gredmedic Co. Ltd., Weihai

264209, China^3

关键词: 16s rDNA sequencing;    Anaerobic bacterium;    Anaerobic cultivation;    Chinese liquors;    Different ages;    High throughput;    Microbial species;    Next-generation sequencing;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/394/2/022025/pdf
DOI  :  10.1088/1757-899X/394/2/022025

来源: IOP

【 摘 要 】

Most Chinese liquor belongs to strong-aroma type, and GujingTribute liquor with a history over 1800 years is one of this type. The flavor of strong-aroma Chinese liquor is largely determined by anaerobic bacteria embedded in the pits, especially the bottom area of the old pits. In this study, culturable anaerobic species in the old (50-100 years old) and young (5 years old) pitmuds were isolated with anaerobic cultivation and characterized by near full-length 16S rDNA sequencing. Ruminococcaceae bacterium CPB6 and Lactobacillus acetotolerans were found as the most culturable species in the freshly-sampled old and young pitmud samples, respectively. This result confirmed our previous findings by high throughput next generation sequencing that Ruminococcaceae bacterium CPB6 was more abundant in good quality pitmud while Lactobacillus acetotolerans was more abundant in ordinary or poor quality pitmud.