Ability of Penicillium Griseofulvum Inacc F 14 in Producing Cellulase Enzyme for Composting Media Plant of White Oyster Mushroom (Pleurotus Ostreatus Jacq. Ex Fr.) P. Kumm and Ear Mushrooms (Auricularia Auricula J.)

Humanosphere Science School 2017; 7th International Symposium for a Sustainable Humanosphere

地球科学;经济学

Soeka, Y.S.^1 ; Ilyas, M.^1

Research Center for Biology, Indonesian Institute of Sciences (LIPI), Jl. Raya Bogor Km. 46, Cibinong, Bogor

16911, Indonesia^1

关键词: 16s rDNA sequencing;    Auricularia auricula;    Carboxymethyl cellulose;    Incubation periods;    Molecular identification;    Optimum conditions;    Pleurotus ostreatus;    Substrate concentrations;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/166/1/012026/pdf
DOI  :  10.1088/1755-1315/166/1/012026

来源: IOP

【 摘 要 】

The aim of this study is to determine the characteristics of cellulase enzymes produced by the fungus LIPIMC selection. Results were then analyzed qualitatively and quantitatively. Enzyme activity includes incubation time, substrate concentration Carboxy Methyl Cellulose (CMC), temperature, optimum pH and the effect of the addition of some metal ions as activators or inhibitors were examined. Further molecular identification of fungus was examined through 16S rDNA sequencing. The optimum conditions for the enzyme activity were the conditions with an incubation period of two days, at pH 6, at the temperature 50°C, at substrate concentration 1.25 %. The cellulase enzyme was activated by cations CoCl2, CuCl2, and NaCl. Meanwhile MgCl2 and ZnCl2 were acted as an inhibitor. Results of molecular identification by 16S rDNA sequencing confirmed that LIPIMC 0575 was Penicillium griseofulvum. After identified, the tested supernatant of cellulase enzyme has the ability to inhibit the growth of Bacillus cereus and will be used for composting on the media plant of oyster mushrooms (Pleurotus ostreatus J.) and ear mushrooms (Auricularia auricula J.)