Microbial Community Composition in a Simultaneous Nitrification and Denitrification Bioreactor for Domestic Wastewater Treatment

2017 2nd International Conference on Environmental Engineering and Sustainable Development

生态环境科学;经济学

Chen, Chen^1 ; Ouyang, Wukun^2 ; Huang, Shan^3 ; Peng, Xiaochun^4

South China Institute of Environmental Sciences, Ministry of Environmental Protection, China^1

Hun School of Princeton, United States^2

Princeton University, United States^3

South China Advanced Environmental Technologies Co., LTD, Guangzhou, China^4

关键词: 16s rDNA sequencing;    Domestic waste water treatment;    Low dissolved oxygen;    Membrane bioreactor;    Microbial community composition;    Simultaneous nitrification and denitrification;    Total Organic Carbon;    Wastewater treatment plants;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/112/1/012007/pdf
DOI  :  10.1088/1755-1315/112/1/012007

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

Traditional domestic wastewater treatments rely on aerobic processes followed by anaerobic processes. The aerobic step in which ammonium and organic carbon are oxidized, calls for large oxygen input, while the anaerobic process often requires extra carbon input. The challenge of synchronizing both processes is to maintain an active nitrifiers sludge under low dissolved oxygen (DO) condition. In this study, a membrane bioreactor was established and operated stable with low DO of 0.1-0.4 mg L-1. Chemical indicators were determined daily, and bacterial community was checked by qPCR and 16S rDNA sequencing every month. After 2 months incubation, the bioreactor reached to a stable removal rate of total nitrogen around 50% and total organic carbon around 90% with the retaining time of 12 h. The sludge showed enrichment of low DO nitrifiers (Nitrosomonadaceae, Chitinophagaceae, and Nitrospiraceae) which were different from sludge in other regular wastewater treatment plants with aerobic and anaerobic cycles.