Dissemination of the high-risk cloneST147 carbapenem-resistant klebsiella pneumoniae from a local tertiary care hospital in the Republic of Korea

Annals of Clinical Microbiology and Antimicrobials

Research

Seongjae Joo¹  Jungsun Park¹  Min-Kyeong Kim¹  Junyoung Kim¹  Hyun Ju Jeong¹  Jaeil Yoo¹  Eunkyung Shin¹  Gwang Rip Hwang²  Jin Seok Kim³ 

[1] Division of Bacterial Diseases, Bureau of Infectious Disease Diagnosis Control, Korea Disease Control and Prevention Agency, Cheongju-si, Chungcheongbuk-do, Republic of Korea;Division of Infectious Diseases Research, Gyeongsangbuk-do Metropolitan Government Research Institute of Public Health and Environment, Gyeongsangbuk-do, Republic of Korea;Infectious Diseases Team, Seoul Metropolitan Government Research Institute of Public Health and Environment, Gwacheon-si, Gyeonggi-do, Republic of Korea;

关键词: Klebsiella pneumoniae;    ST147;    Carbapenemase;    NDM-1;   

DOI  :  10.1186/s12941-023-00601-2

 received in 2023-01-09, accepted in 2023-05-29,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundThe emergence of carbapenem-resistant Enterobacterales (CRE) infections is rapidly increasing and represents a serious public threat. In 2020, a total of 16,883 carbapenemase-producing Enterobacterales strains were collected; among these isolates, 21 strains were repeatedly isolated in a local tertiary care hospital.MethodsAntimicrobial susceptibility testing was performed using the broth microdilution method. All 21 strains of CRKP were analyzed by PFGE after XbaI digestion. The 21 CRKP strains were sequenced on the Illumina Miseq and Oxford Nanopore GridION platforms.ResultsThese 21 CRKP isolates showed an identical antimicrobial resistance profile, including resistance to ampicillin, carbapenems, cephems, chloramphenicol, fluoroquinolone, macrolides and trimethoprim/sulfamethoxazole. Based on whole-genome analysis, these 21 CRKP isolates shared a common genetic structure (ISAba125-IS630-blaNDM−1-bleMBL) and harbored additional resistance determinants (blaOXA−1, blaCTX−M−15, blaSHV−11, blaSHV−67, aac(6’)-Ib-cr, qnrS1, OqxA, OqxB, catB3, mph(A), sul1, and dfrA12) and mutations in the quinolone resistance-determining regions of gyrA (S83I) and parC (S80I). These isolates belonged to the ST147 and KL64 capsular types, which were carried on IncFIB replicon plasmids. The 21 CRKP strains collected from one hospital were divided into five PFGE patterns, and they were closely related with a minimum similarity value of 95.2%. These isolates were found to be highly related based on the presence of between 2 and 27 SNPs.ConclusionsThese findings indicate that NDM-1-producing K. pneumoniae ST147 may have been introduced via a common source, implying nosocomial transmission; furthermore, continuous monitoring is necessary to prevent endemic transmission.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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