Determination quercetin content, antioxidant and antimicrobial activity of genotype mutant Samosir shallots irradiated by gamma rays

International Conference on Agriculture, Environment, and Food Security

农业科学;生态环境科学

Sinuraya, M.^1 ; Hanafiah, D.S.^1 ; Romulo, A.^2 ; Barus, A.^1

Faculty of Agriculture, Universitas Sumatera Utara, Medan

20155, Indonesia^1

Department of Crop Sciences and Agroforestry, Faculty of Tropical Agrisciences, Czech Universuty of Life Sciences, Prague, Suchdol, 6, Czech Republic^2

关键词: Anti-microbial activity;    Anti-oxidant activities;    Bacillus Subtilis;    Control samples;    Enterococcus faecalis;    Klebsiella pneumoniae;    Long-term storage;    Pseudomonas aeruginosa;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/122/1/012070/pdf
DOI  :  10.1088/1755-1315/122/1/012070

来源: IOP

【 摘 要 】

The aim of the research was to study the variation in antioxidant and antimicrobial activity as well as the total quercetin content of the fifth generation genotypes mutant Samosir shallot irradiated by gamma rays. The studies conducted included the assessment of quercetin content, antioxidant and antimicrobial activity in shallot bulbs after long-term storage (6 months in the room temperature). Quercetin content of 20 selected genotype mutants of irradiated shallot bulbs along with untreated populations were calculated using quercetin (QU) as a standard. Antioxidant activities of 8 genotype mutant were determined using DPPH. Antimicrobial activity of bulb extracts were tested against six bacteria including Staphylococcus aurous, Enterococcus faecalis, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae and oneyeastCandida albicans. The results showed that population of genotype mutants irradiated with dosage 2Gy, 4 Gy, 5 Gy and 6 Gy have higher quercetin content than control samples. None of the genotype mutants exhibited antibacterial inhibitory against all microorganism tested except for the sample number 2 and 6 (bulbs generated from the plants irradiated by gamma rays with dosage at 2 Gy and 6 Gy). There was also none of the genotypes observed exhibited significant antioxidant efficacy.