Antimicrobial activity of tempeh gembus hydrolyzate

3rd International Conference on Tropical and Coastal Region Eco Development 2017

地球科学;生态环境科学

Noviana, A.^1 ; Dieny, F.F.^1,2 ; Rustanti, N.^1,2 ; Anjani, G.^1,2 ; Afifah, D.N.^1,2

Department of Nutrition Science, Faculty of Medicine, Diponegoro University, Jl. Prof. H. Soedarto, SH, Tembalang, Semarang

50275, Indonesia^1

Center of Nutrition Research (CENURE), Diponegoro University, Jl. Prof. H. Soedarto, SH, Tembalang, Semarang

50275, Indonesia^2

关键词: Anti-microbial activity;    Anti-microbial effects;    Anti-microbial tests;    Bacillus Subtilis;    Hydrolyzate;    Staphylococcus aureus;    Tempeh Gembus;    Tropical disease;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/116/1/012044/pdf
DOI  :  10.1088/1755-1315/116/1/012044

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

Tropical disease can be prevented by consumming fermented foods that have antimicrobial activity. One of them is tempeh gembus that has short shelf life. It can be overcome by processing it into hydrolyzate. This study aimed to determine antimicrobial activity of tempeh gembus hydrolyzate. Tempeh gembus was made of local soybean from Grobogan. They were added 5,000 ppm, 8,000 ppm, and 10,000 ppm of bromelain enzyme (TGH BE). Antimicrobial effects of TGH BE were tested against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Steptococcus mutans. Antimicrobial test was carried out using Kirby-Bauer Disc Diffussion method. Soluble protein test used Bradford method. The largest inhibition zone against S. aureus and S. mutans were shown by TGH BE 8,000 ppm, 0.89±0.53 mm and 2.40±0.72 mm. The largest inhibition zone of B. subtilis, 7.33±2,25 mm, was shown by TGH BE 5,000 ppm. There wasn't antimicrobial effect of TGH BE against E. coli. There weren't significant differences of soluble protein (P=0.293) and the inhibition zones againt S. aureus (P = 0.967), E. coli (P = 1.000), B. subtilis (P = 0.645), S. mutans (P=0.817) of all treatments. There were antimicrobial activities of TGH BE against S. aureus, B. subtilis, and S. mutans.