Phenotypic and molecular detection of BLACTX-M gene extended-spectrum beta-lactamases in escherichia coli and klebsiella pneumoniae of north sumatera isolates

3rd International Conference on Biological Sciences and Biotechnology

Hasibuan, Mirzan^1,2 ; Suryanto, Dwi^1 ; Kusumawati, R Lia^2,3,4

Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sumatera Utara, Kampus USU, Jln. Bioteknologi No. 1, Medan

20155, Indonesia^1

Clinical Research Unit, Clinical Microbiology Unit, University of Sumatera Utara Hospital, Kampus USU, Jln. Dr. T Mansur No. 66, Medan

20154, Indonesia^2

Department of Microbiology, Faculty of Medicine, University of Sumatera Utara, Kampus USU, Jln. Universitas No.1, Medan

20155, Indonesia^3

H. Adam Malik Hospital Medan, Indonesia^4

关键词: Antibiotic resistance;    Bacterial antibiotic resistance;    ESBLs;    infections;    Infectious disease;    Klebsiella pneumoniae;    Molecular detection;    Polymerase chain reaction techniques;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/130/1/012032/pdf
DOI  :  10.1088/1755-1315/130/1/012032

来源: IOP

【 摘 要 】

The application of antibiotics expanded-spectrum third-generation cephalosporin for the treatment of infectious diseases in hospitals is known contribute to increasing resistance due to the presence of theblaCTX-M gene in the bacteria producing ESBLs. This study was aimed to detect ESBLs, isolate phenotype andblaCTX-M genes on Escherichia coli and Klebsiella pneumoniae collected from H. Adam Malik Central Hospital. Phenotypes of the bacterial were detection using Vitek two compact, while theblaCTX-M genes were detection using polymerase chain reaction technique. The results showed that 85 (100%) isolates were ESBLs consisted of 41(48%) of Escherichia coli, and 44 (52%) of Klebsiella pneumoniae, respectively.blaCTX-M genes were detection in 62 (72.94%) of the isolates which 31 (36.47%) were Escherichia coli, and 31 (36.47%) of the isolates were Klebsiella pneumoniae, respectively. This study indicates the high prevalence ofblaCTX-M genes in Escherichia coli and Klebsiella pneumoniea causing bacterial antibiotic resistance.