A global multinational survey of cefotaxime-resistant coliforms in urban wastewater treatment plants

Environment International

Bastian Herzog¹  Shichun Zou¹  Leonie Henn²  Pawel Krzeminski³  Telma Fernandes⁴  Stella G. Michael⁵  Veljo Kisand⁶  Pilar Caballero⁷  Sara Rodriguez-Mozaz⁷  Yunho Lee⁸  Heidrun Mayrhofer⁸  Joshua T. Bunce⁹  Edouard Jurkevitch^1,10  Katarzyna Slipko^1,10  Marta Piotrowska^1,11  Marcos Quintela-Baluja^1,11  Roberto Rosal^1,12  Despo Fatta-Kassinos^1,12  Barbara Drigo^1,13  Erica Donner^1,13  Ester Heath^1,14  Anna Baraniak^1,14  Eddie Cytryn^1,15  Connor Brown^1,15  Virginia Riquelme^1,16  Christophe Merlin^1,16  Amy Pruden^1,16  Andrew Scott^1,17  Roberto B.M. Marano^1,17  Marta Tacão^1,18  Isabel Henriques^1,18  Ana Agüera^1,19  Belen Esteban^1,19  Samira Nahim–Granados^2,20  Maria I. Polo-López^2,20  Agnieszka Kalinowska^2,21  Aneta Luczkiewicz^2,21  Magdalena Popowska^2,22  Isabel Martínez-Alcalá^2,23  Jose M. Guillén-Navarro^2,23  Thor A. Stenstrom^2,24  Anthony A. Adegoke^2,24  Hemda Garelick^2,25  Karin Beck^2,25  Hélène Guilloteau^2,26  Carsten U. Schwermer^2,26  Manika Choudhury^2,27  Joana Abreu-Silva^2,27  Norbert Kreuzinger^2,28  Mailis Laht^2,28  Milena Milakovic^2,29  Marie-Noëlle Pons^2,29  Gianluca Corno^3,30  Popi Karaolia^3,30  Alice L. Petre^3,30  Donald Morrison^3,31  Ying Yang^3,32  Younggun Yoon^3,32  Om Prakash^3,33  Yogesh Nimonkar^3,33  Marco Guida^3,34  Giovanni Libralato^3,34  Thomas Jäger^3,35  Thomas Schwartz^3,35  Stefanie Heß^3,36  Thomas U. Berendonk^3,36  Ayella Maile-Moskowitz^3,37  Gianluca Brunetti^3,37  Nikolina Udikovic-Kolic^3,38  Jérôme Ory^3,38  Stela Krizanovic^3,39  Leonardo P. Munoz^3,39  William H. Gaze^4,40  Antoni Oliver^4,40  Anne Leonard^4,40  Traore Ousmane^4,40  Edward Topp^4,41  Helmut Bürgmann^4,41  Olga Nunes^4,42  Célia M. Manaia^4,42  Carlo Salerno^4,43  Alfieri Pollice^4,43  Jose L. Balcazar^4,44  Andrea Di Cesare^4,45  Tanel Telson^4,45 

[1] CNRS, UMR 6023, LMGE, F-63170 Campus Universitaire des Cézeaux, Clermont-Ferrand, France;Department of Biology, University of Western Ontario, London, ON, Canada;Department of Microbiology, University of Uyo, Uyo, Nigeria;Department of Soil Chemistry, Plant Nutrition and Microbiology, Institute of Soil Water and Environmental Sciences, Volcani Center, Agricultural Research Organization, Rishon Lezion, Israel;Estonian Environmental Research Centre, Estonia;Institute of Microbiology, TU Dresden, Dresden, Germany;Plataforma Solar de Almería – CIEMAT, P.O. Box 22, 04200 Tabernas, Almería, Spain;Service d'hygiène hospitalière, CHU Clermont-Ferrand, Clermont-Ferrand, France;University of Coimbra, Department of Life Sciences, Calçada Martim de Freitas, 3000-456 Coimbra, Portugal;Agriculture and Agri-Food Canada, London Research and Development Centre (ON), Canada;CESAM and Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193, Portugal;CNR-IRSA Molecular Ecology Group, Largo Tonolli 50, 28922 Verbania, Italy;CNR-IRSA Viale F. De Blasio 5, 70132 Bari, Italy;Catalan Institute for Water Research (ICRA), 17003 Girona, Spain;Chair of Urban Water Systems Engineering, Technical University of Munich (TUM), Germany;Civil and Environmental Engineering Department and Nireas International Water Research Center, University of Cyprus, P.O. Box 20537, CY-1678 Nicosia, Cyprus;Department of Agroecology and Plant Health, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel;Department of Biology, University of Naples Federico II, via Cinthia 21, 80126 Naples, Italy;Department of Chemical Engineering, University of Alcalá, E-28871 Alcalá de Henares, Madrid, Spain;Department of Civil Engineering, Av. de los Jerónimos, 135, 30107 Guadalupe, Murcia, Spain;Department of Civil and Environmental Engineering, Virginia Tech, Blacksburg, VA, USA;Department of Natural Sciences, Middlesex University, London NW4 4BT, UK;Eawag, Swiss Federal Institute of Aquatic Science and Technology, 6047 Kastanienbaum, Switzerland;Faculty of Civil and Environmental Engineering, Gdansk University of Technology, G. Narutowicza 11/12 street, 80-233 Gdańsk, Poland;Future Industries Institute, University of South Australia, Adelaide, SA 5001, Australia;Institute for Water and Wastewater Technology, Durban University of Technology, Durban South Africa;Institute of Functional Interfaces (IFG), Karlsruhe Institute of Technology (KIT), Eggenstein-Leopoldshafen, Germany;Institute of Hydrobiology, TU Dresden, Dresden, Germany;Institute of Microbiology, Department of Applied Microbiology, Faculty of Biology, University of Warsaw, Poland;Institute of Technology, University of Tartu, Estonia;LEPABE, Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal;Laboratoire “Microorganisme: Génome et Environnement”, Université Clermont Auvergne, BP 10448, F-63000 Clermont-Ferrand, France;Laboratori EMATSA, Ctra Valls Km 3, 43130 Tarragona, Spain;Norwegian Institute for Water Research, Gaustadalléen 21, N-0349 Oslo, Norway;Rudjer Boskovic Institute, Bijenicka 54, Zagreb, Croatia;School Applied Sciences, Edinburgh Napier University, EH11 4BN, UK;School of Earth Sciences and Environmental Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea;School of Engineering, Newcastle University, Newcastle Upon Tyne, UK;School of Marine Sciences, Sun Yat-sen University, Guangzhou, China;Solar Energy Research Centre (CIESOL), Joint Centre University of Almería-CIEMAT, 04120 Almería, Spain;UMR 7564 University of Lorraine-CNRS (LCPME), France;Universidade Católica Portuguesa, CBQF – Centro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superior de Biotecnologia, Rua Arquiteto Lobão Vital, 172, 4200-374 Porto, Portugal;University of Exeter Medical School, European Centre for Environment and Human Health, Environment and Sustainability Institute, University of Exeter, Penryn campus, TR10 9FE, UK;Université de Lorraine, CNRS, LRGP, F-54000 Nancy, France;Vienna University of Technology, Institute for Water Quality and Resources Management, Vienna, Austria;

关键词: Antibiotic resistance;    Coliforms;    ESBLs;    Wastewater treatment;    Water reuse;   

DOI  :  

来源: DOAJ

【 摘 要 】

The World Health Organization Global Action Plan recommends integrated surveillance programs as crucial strategies for monitoring antibiotic resistance. Although several national surveillance programs are in place for clinical and veterinary settings, no such schemes exist for monitoring antibiotic-resistant bacteria in the environment. In this transnational study, we developed, validated, and tested a low-cost surveillance and easy to implement approach to evaluate antibiotic resistance in wastewater treatment plants (WWTPs) by targeting cefotaxime-resistant (CTX-R) coliforms as indicators. The rationale for this approach was: i) coliform quantification methods are internationally accepted as indicators of fecal contamination in recreational waters and are therefore routinely applied in analytical labs; ii) CTX-R coliforms are clinically relevant, associated with extended-spectrum β-lactamases (ESBLs), and are rare in pristine environments. We analyzed 57 WWTPs in 22 countries across Europe, Asia, Africa, Australia, and North America. CTX-R coliforms were ubiquitous in raw sewage and their relative abundance varied significantly (<0.1% to 38.3%), being positively correlated (p < 0.001) with regional atmospheric temperatures. Although most WWTPs removed large proportions of CTX-R coliforms, loads over 103 colony-forming units per mL were occasionally observed in final effluents. We demonstrate that CTX-R coliform monitoring is a feasible and affordable approach to assess wastewater antibiotic resistance status.

【 授权许可】

Unknown