Bacterial reduction of mature Enterococcus faecalis biofilm by different irrigants and activation techniques using confocal laser scanning microscopy. An in vitro study

Saudi Endodontic Journal

article

Ameera Lotfy Mahfouze¹  Abeer A. Elhakim El Gendy²  Tarek Medhat Elsewify² 

[1] Department of Endodontics, Faculty of Dentistry, October Six University;Department of Endodontics, Faculty of Dentistry, Ain Shams University

关键词: Bacterial biofilm;    confocal laser scanning microscopy;    irrigating solution;    sonic;    ultrasonic;   

DOI  :  10.4103/sej.sej_135_19

学科分类：口腔科学

来源: Medknow

【 摘 要 】

Introduction: The aim of this study was to evaluate the effect of ultrasonic and sonic activation using three irrigants on mature Enterococcus faecalis biofilm. Materials and Methods: Seventy single-rooted premolars were prepared and sterilized. Mature E. faecalis biofilm was developed. Roots were randomly divided into three groups (n = 21) according to activation technique: ultrasonic, sonic, and positive control. Each group was further subdivided into three subgroups (n = 7) according to the irrigant used: 4% propolis, 2% chlorhexidine (CHX), and 2.5% sodium hypochlorite. Samples were cut and scanned using confocal laser scanning microscopy. The fluorescent images were analyzed using Zen imaging software. Data analysis was performed using one way analysis of variance and Tukey's honestly significant difference test for pairwise comparison. Statistical significance was set at 5%. Results: Both activated groups showed a statistically significant bacterial reduction (P ≤ 0.001). CHX showed the highest antibacterial effect. Conclusions: Irrigant activation is an essential step in reduction of bacterial counts. CHX has a potent antibacterial effect against mature E. faecalis biofilm.

【 授权许可】

CC BY-NC-SA