Histological study of the effect of some irrigating solutions on bacterial endotoxin in dogs

Brazilian Dental Journal

Léa Assed Bezerra Da Silva²  Mario Roberto Leonardo¹  Sada Assed²  Mário Tanomaru Filho¹ 

[1] ,USP School of Dentistry of Ribeirão Preto Department of Clinical PediatricsRibeirão Preto SP ,Brazil

关键词: endotoxin;    root canal therapy;    irrigating solution;   

DOI  :  10.1590/S0103-64402004000200005

来源: SciELO

【 摘 要 】

The aim of this study was to evaluate, histopathologically, the effectiveness of mechanical preparation of root canals using different irrigating solutions in dog teeth filled with LPS after pulpectomy. A total of 120 root canals of 6 mongrel dogs were filled with a solution of LPS after pulpectomy. The irrigating solutions used were saline, 1, 2.5, and 5% sodium hypochlorite, and 2% chlorhexidine. No irrigation was used in the control group. The animals were sacrificed after 60 days and the teeth were fixed and demineralized. Subsequently, serial 6-µm sections were stained with hematoxylin and eosin and Mallory's trichrome for histopathological analysis and Brown-Brenn for verification of bacterial contamination. Analysis showed that the inflammatory infiltrate was statistically less intense in the groups in which the root canals were irrigated with 5% sodium hypochlorite and 2% chlorhexidine. However, none of the irrigating solutions completely inactivated the harmful effects of LPS. Mechanical preparation associated with different irrigating solutions did not completely inactivate LPS.

【 授权许可】

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