Silver nanoparticles influence on the blood activation process and their release to blood plasma from synthetic polymer scaffold

International Conference on Materials, Processing and Product Engineering 2015

Major, R.^1 ; Lackner, J.M.^2 ; Sanak, M.^3 ; Major, B.^1

Institute of Metallurgy and Materials Science, Polish Academy of Sciences, Reymonta St. 25, Crakow, Poland^1

Joanneum Research Forschungs-GmbH, Materials - Functional Surfaces, Leoben, Austria^2

Department of Medicine, Jagiellonian University Medical College, 8 Skawinska Street, Cracow

31-066, Poland^3

关键词: Activated partial thromboplastin time;    Activation process;    Bacterial biofilm;    Biomedical treatment;    Crystalline solids;    Hetero interfaces;    Prothrombin time;    Synthetic polymer scaffolds;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/119/1/012031/pdf
DOI  :  10.1088/1757-899X/119/1/012031

来源: IOP

【 摘 要 】

In the present work, blood and blood plasma interaction to silver stabilised polyelectrolytes was investigated in vitro. The designed materials are dedicated for regeneration of the cardiovascular system. Silver nanoparticles were introduced into the polyelectrolyte structure in order to reduce the risk of bacterial biofilm formation. The introduction of Ag nanoparticles occurred by deposition at high vacuum by magnetron sputtering. The analysis of blood-materials interactions were performed by using commercially available tester, Impact-R (Diamed). The assessment of silver ion nanoparticles release into the plasma consisted in determining the Prothrombin Time (PT) and Activated Partial Thromboplastin Time (APTT). Unmodified surface of polyelectrolytes is a strong activator for blood elements. The introduction of silver nanoparticles resulted in a significant reduction in the probability of clotting. The extrinsic pathway of coagulation determined on the basis of the PT and the intrinsic and common pathways of coagulation measured by the APTT did not indicate the danger out of range. Microstructure was studied using TEM on thin foils prepared from the cross-section of samples subjected to biomedical treatments. The observations revealed hetero- interface between two different crystalline solids.