Antioxidant and Apoptotic Effect of Muscari muscarimi, an Endemic Geophyte Species from Turkey

Jordan Journal of Biological Sciences

article

Cennet Ozay¹  Ege Riza Karagur²  Ramazan Mammadov³  Hakan Akca² 

[1] Izmir Katip Celebi University, Faculty of Pharmacy, Department of Basic Pharmaceutical Sciences;Pamukkale University, Faculty of Medicine, Department of Medical Genetics;Muğla Sıtkı Koçman University, Faculty of Science, Department of Molecular Biology and Genetics

关键词: Antioxidant activity;    Apoptosis;    Cancer cell lines;    Muscari muscarimi;    Turkey;   

DOI  :  10.54319/jjbs/140424

学科分类：生物科学（综合）

来源: Hashemite University, Deanship of Research and Higher Studies

【 摘 要 】

Muscari muscarimi Medik., which is an endangered and endemic geophyte species of Turkey, was studied for antioxidant, cytotoxic and apoptotic potentials. The antioxidant effect of the extracts (ethanol and methanol) provided from the flower and bulbs of M. muscarimi was tested through the antioxidant potency by phosphomolybdenum assay, the reducing power assay, metal chelating ability, nitric oxide scavenging and ABTS radical cation scavenging capability methods. Also, total flavonoid and saponin content of the extracts was specified. Cytotoxic activity of the extracts was assessed and screened against human MCF-7, HeLa and H1299 cancer cells. Terminal transferase dUTP nick end-labeling (TUNEL) assay was applied to cancer cells for the determination of the late apoptotic changes. Antioxidant potencies in bulb extracts were observed to be lower than the flower extracts. However, the cytotoxicity and TUNEL assay revealed that the bulb extracts exhibited more marked anticancer activity against H1299 cell line than the other cell lines. Based on the in vitro data, M. muscarimi warrants further studies to isolate novel compounds for chemotherapeutic use.

【 授权许可】

CC BY