期刊论文详细信息
European Journal of Medical Research
Autophagy impairment in patients with obstructive sleep apnea modulates intermittent hypoxia-induced oxidative stress and cell apoptosis via hypermethylation of the ATG5 gene promoter region
Research
Chia-Wei Liou1  Te-Yao Hsu2  I-Chun Lin3  Ting-Ya Wang4  Chiu-Ping Lee4  Po-Yuan Hsu4  Yong-Yong Lin4  Yung-Che Chen5  Meng-Chih Lin5  Chang-Chun Hsiao6  Yu-Mu Chen7  Chien-Hung Chin7  Mao-Chang Su8  Jen-Chieh Chang9 
[1] Department of Neurology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, 83301, Kaohsiung, Taiwan;Department of Obstetrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, 83301, Kaohsiung, Taiwan;Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, 83301, Kaohsiung, Taiwan;Division of Pulmonary and Critical Care Medicine, Department of Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, No. 123, Ta-Pei Rd, Niao-Sung District, 83301, Kaohsiung, Taiwan;Division of Pulmonary and Critical Care Medicine, Department of Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, No. 123, Ta-Pei Rd, Niao-Sung District, 83301, Kaohsiung, Taiwan;Department of Medicine, College of Medicine, Chang Gung University, 33302, Taouyan, Taiwan;Sleep Center, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, No. 123, Ta-Pei Rd, Niao-Sung District, 83301, Kaohsiung, Taiwan;Division of Pulmonary and Critical Care Medicine, Department of Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, No. 123, Ta-Pei Rd, Niao-Sung District, 83301, Kaohsiung, Taiwan;Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, 33302, Taouyan, Taiwan;Division of Pulmonary and Critical Care Medicine, Department of Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, No. 123, Ta-Pei Rd, Niao-Sung District, 83301, Kaohsiung, Taiwan;Sleep Center, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, No. 123, Ta-Pei Rd, Niao-Sung District, 83301, Kaohsiung, Taiwan;Division of Pulmonary and Critical Care Medicine, Department of Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, No. 123, Ta-Pei Rd, Niao-Sung District, 83301, Kaohsiung, Taiwan;Sleep Center, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, No. 123, Ta-Pei Rd, Niao-Sung District, 83301, Kaohsiung, Taiwan;Chang Gung University of Science and Technology, 61363, Chiayi, Taiwan;Genomics and Proteomics Core Lab, Department of Medical Research, Kaohsiung Chang Gung Memorial Hospital, 83301, Kaohsiung, Taiwan;
关键词: Obstructive sleep apnea;    Autophagy;    DNA methylation;    Mesenchymal stem cell;    Intermittent hypoxia with re-oxygenation;    Rapamycin;   
DOI  :  10.1186/s40001-023-01051-4
 received in 2022-09-01, accepted in 2023-02-07,  发布年份 2023
来源: Springer
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【 摘 要 】

BackgroundAutophagy is a catabolic process that recycles damaged organelles and acts as a pro-survival mechanism, but little is known about autophagy dysfunction and epigenetic regulation in patients with obstructive sleep apnea (OSA).MethodsProtein/gene expressions and DNA methylation levels of the autophagy-related genes (ATG) were examined in blood leukocytes from 64 patients with treatment-naïve OSA and 24 subjects with primary snoring (PS).ResultsLC3B protein expression of blood monocytes, and ATG5 protein expression of blood neutrophils were decreased in OSA patients versus PS subjects, while p62 protein expression of cytotoxic T cell was increased, particularly in those with nocturia. ATG5, ULK1, and BECN1 gene expressions of peripheral blood mononuclear cells were decreased in OSA patients versus PS subjects. LC3B gene promoter regions were hypermethylated in OSA patients, particularly in those with excessive daytime sleepiness, while ATG5 gene promoter regions were hypermethylated in those with morning headache or memory impairment. LC3B protein expression of blood monocytes and DNA methylation levels of the LC3B gene promoter region were negatively and positively correlated with apnea hyponea index, respectively. In vitro intermittent hypoxia with re-oxygenation exposure to human THP-1/HUVEC cell lines resulted in LC3B/ATG5/ULK1/BECN1 down-regulations and p62 up-regulation along with increased apoptosis and oxidative stress, while rapamycin and umbilical cord-mesenchymal stem cell treatment reversed these abnormalities through de-methylation of the ATG5 gene promoter.ConclusionsImpaired autophagy activity in OSA patients was regulated by aberrant DNA methylation, correlated with clinical phenotypes, and contributed to increased cell apoptosis and oxidative stress. Autophagy enhancers may be novel therapeutics for OSA-related neurocognitive dysfunction.

【 授权许可】

CC BY   
© The Author(s) 2023

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