The autophagy GABARAPL1 gene is epigenetically regulated in breast cancer models

BMC Cancer

Research Article

Marie-Paule Algros¹  Franck Monnien¹  Régis Delage-Mourroux²  Eric Hervouet²  Thierry Gauthier²  Pascale Adami²  Aurore Claude-Taupin²  Valérie Perez²  Annick Fraichard²  Gilles Despouy²  Michèle Jouvenot²  Michaël Boyer-Guittaut² 

[1] Department of Pathology, University Hospital Jean-Minjoz, 25030, Besançon, France;Université de Franche-Comté, Laboratoire de Biochimie, EA3922 « Estrogènes, Expression Génique et Pathologies du Système Nerveux Central », SFR IBCT FED4234, UFR Sciences et Techniques, 16 route de Gray, 25030, Besançon Cedex, France;

关键词: Autophagy;    GABARAP;    GABARAPL1;    GABARAPL2;    Breast cancer;    CREB-1;    DNA methylation;    Epigenetics;   

DOI  :  10.1186/s12885-015-1761-4

 received in 2015-05-05, accepted in 2015-10-09,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThe GABARAP family members (GABARAP, GABARAPL1/GEC1 and GABARAPL2 /GATE-16) are involved in the intracellular transport of receptors and the autophagy pathway. We previously reported that GABARAPL1 expression was frequently downregulated in cancer cells while a high GABARAPL1 expression is a good prognosis marker for patients with lymph node-positive breast cancer.MethodsIn this study, we asked using qRT-PCR, western blotting and epigenetic quantification whether the expression of the GABARAP family was regulated in breast cancer by epigenetic modifications.ResultsOur data demonstrated that a specific decrease of GABARAPL1 expression in breast cancers was associated with both DNA methylation and histone deacetylation and that CREB-1 recruitment on GABARAPL1 promoter was required for GABARAPL1 expression.ConclusionsOur work strongly suggests that epigenetic inhibitors and CREB-1 modulators may be used in the future to regulate autophagy in breast cancer cells.

【 授权许可】

CC BY   
© Hervouet et al. 2015