期刊论文详细信息
BMC Cancer
The autophagy GABARAPL1 gene is epigenetically regulated in breast cancer models
Research Article
Marie-Paule Algros1  Franck Monnien1  Régis Delage-Mourroux2  Eric Hervouet2  Thierry Gauthier2  Pascale Adami2  Aurore Claude-Taupin2  Valérie Perez2  Annick Fraichard2  Gilles Despouy2  Michèle Jouvenot2  Michaël Boyer-Guittaut2 
[1] Department of Pathology, University Hospital Jean-Minjoz, 25030, Besançon, France;Université de Franche-Comté, Laboratoire de Biochimie, EA3922 « Estrogènes, Expression Génique et Pathologies du Système Nerveux Central », SFR IBCT FED4234, UFR Sciences et Techniques, 16 route de Gray, 25030, Besançon Cedex, France;
关键词: Autophagy;    GABARAP;    GABARAPL1;    GABARAPL2;    Breast cancer;    CREB-1;    DNA methylation;    Epigenetics;   
DOI  :  10.1186/s12885-015-1761-4
 received in 2015-05-05, accepted in 2015-10-09,  发布年份 2015
来源: Springer
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【 摘 要 】

BackgroundThe GABARAP family members (GABARAP, GABARAPL1/GEC1 and GABARAPL2 /GATE-16) are involved in the intracellular transport of receptors and the autophagy pathway. We previously reported that GABARAPL1 expression was frequently downregulated in cancer cells while a high GABARAPL1 expression is a good prognosis marker for patients with lymph node-positive breast cancer.MethodsIn this study, we asked using qRT-PCR, western blotting and epigenetic quantification whether the expression of the GABARAP family was regulated in breast cancer by epigenetic modifications.ResultsOur data demonstrated that a specific decrease of GABARAPL1 expression in breast cancers was associated with both DNA methylation and histone deacetylation and that CREB-1 recruitment on GABARAPL1 promoter was required for GABARAPL1 expression.ConclusionsOur work strongly suggests that epigenetic inhibitors and CREB-1 modulators may be used in the future to regulate autophagy in breast cancer cells.

【 授权许可】

CC BY   
© Hervouet et al. 2015

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