Genomic and phenotypic profiling of Staphylococcus aureus isolates from bovine mastitis for antibiotic resistance and intestinal infectivity

BMC Microbiology

Research

Satwik Majumder¹  Saji George¹  Trisha Sackey¹  Soyoun Park¹  Jennifer Ronholm²  Charles Viau³  Jianguo Xia⁴ 

[1]Department of Food Science and Agricultural Chemistry, Macdonald Campus, McGill University, Macdonald-Stewart Building, Room-1039, 21, 111 Lakeshore Ste Anne de Bellevue, H9X 3V9, Quebec, Canada

[2]Department of Food Science and Agricultural Chemistry, Macdonald Campus, McGill University, Macdonald-Stewart Building, Room-1039, 21, 111 Lakeshore Ste Anne de Bellevue, H9X 3V9, Quebec, Canada

[3]Department of Animal Science, Macdonald Campus, McGill University, 21, 111 Lakeshore Ste Anne de Bellevue, H9X 3V9, Quebec, Canada

[4]Institute of Parasitology, Macdonald Campus, McGill University, 21, 111 Lakeshore Ste Anne de Bellevue, H9X 3V9, Quebec, Canada

[5]Institute of Parasitology, Macdonald Campus, McGill University, 21, 111 Lakeshore Ste Anne de Bellevue, H9X 3V9, Quebec, Canada

[6]Department of Animal Science, Macdonald Campus, McGill University, 21, 111 Lakeshore Ste Anne de Bellevue, H9X 3V9, Quebec, Canada

关键词: Staphylococcus aureus;    Bovine mastitis;    Antibiotics;    Antibiotic resistance (ABR);    Virulence characteristics;    Intracellular pathogens;    Zoonotic spillover;    Intestinal infection;    Caco-2 cells;    Caenorhabditis elegans;   

DOI  :  10.1186/s12866-023-02785-1

 received in 2022-08-18, accepted in 2023-01-30,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundStaphylococcus aureus is one of the prevalent etiological agents of contagious bovine mastitis, causing a significant economic burden on the global dairy industry. Given the emergence of antibiotic resistance (ABR) and possible zoonotic spillovers, S aureus from mastitic cattle pose threat to both veterinary and public health. Therefore, assessment of their ABR status and pathogenic translation in human infection models is crucial.ResultsIn this study, 43 S. aureus isolates associated with bovine mastitis obtained from four different Canadian provinces (Alberta, Ontario, Quebec, and Atlantic provinces) were tested for ABR and virulence through phenotypic and genotypic profiling. All 43 isolates exhibited crucial virulence characteristics such as hemolysis, and biofilm formation, and six isolates from ST151, ST352, and ST8 categories showed ABR. Genes associated with ABR (tetK, tetM, aac6’, norA, norB, lmrS, blaR, blaZ, etc.), toxin production (hla, hlab, lukD, etc.), adherence (fmbA, fnbB, clfA, clfB, icaABCD, etc.), and host immune invasion (spa, sbi, cap, adsA, etc.) were identified by analyzing whole-genome sequences. Although none of the isolates possessed human adaptation genes, both groups of ABR and antibiotic-susceptible isolates demonstrated intracellular invasion, colonization, infection, and death of human intestinal epithelial cells (Caco-2), and Caenorhabditis elegans. Notably, the susceptibilities of S. aureus towards antibiotics such as streptomycin, kanamycin, and ampicillin were altered when the bacteria were internalized in Caco-2 cells and C. elegans. Meanwhile, tetracycline, chloramphenicol, and ceftiofur were comparatively more effective with ≤ 2.5 log10 reductions of intracellular S. aureus.ConclusionsThis study demonstrated the potential of S. aureus isolated from mastitis cows to possess virulence characteristics enabling invasion of intestinal cells thus calling for developing therapeutics capable of targeting drug-resistant intracellular pathogens for effective disease management.

【 授权许可】

CC BY   
© The Author(s) 2023

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