Synthesis of Graphene Oxide Using Simplified Hummer's Method for Antibacterial Application

2nd International Conference on Sustainable Engineering Techniques

工业技术（总论）

Mohammad, M.R.^1 ; Abdul Hussien, Adi M.^1 ; Ghanim, Russel R.^1

Department of Applied Science, University of Technology, Baghdad, Iraq^1

关键词: Anti-bacterial activity;    Biological applications;    Colony forming units;    Escherichia coli (E. coli);    Gram-positive bacterium;    Simplified Hummer's Method;    Staphylococcus aureus;    Structural and morphological properties;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/518/6/062012/pdf
DOI  :  10.1088/1757-899X/518/6/062012

学科分类：工业工程学

来源: IOP

【 摘 要 】

In order to promote the biological applications of graphene based materials, great exploration for nano-scale preparation of graphene oxide (GO) has been made with a novel facile processing method and low cost. For these reasons this method has been carried out in the following study. The GO nanosheets have been prepared depending on Hummer's method, which represents an easy and efficient technique since 1958. Alternations have been done to simplify this method in order to prepare the GO with a novel way without a need to add NaNO3. Also this preparation has been achieved at room temperature without a need to water bath at temperatures of 35 °C and 98 °C. Structural and morphological properties and contents of the synthesis nanosheets were characterized by using XRD, SEM, and EDS techniques, respectively. The purpose of this work is to evaluate the antibacterial activity of GO nansheets against Gram-negative and Gram-positive bacteria of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), respectively. Bacteriological test were achieved by colony forming units (CFUs) assay. CFUs assay has ensured potential of GO as an anti-infective agent for controlling the growth of two spices of bacteria. The results have showed that Gram-positive were more effective by nanosheets than Gram-negative.