Inhibition of fish bacteria pathogen in tilapia using a concoction three of Borneo plant extracts

1st International Conference on Tropical Studies and Its Application

Hardi, E.H.^1 ; Saptiani, G.^1 ; Kusuma, I.W.^2 ; Suwinarti, W.^2 ; Sudaryono, A.^3

Department of Aquaculture, Faculty of Fisheries and Marine Science, Mulawarman University, Jl. Gunung Tabur, GunungKelua, Samarinda Ulu, Samarinda, East Kalimantan

75123, Indonesia^1

Faculty of Forestry, Mulawarman University, Samarinda, East Kalimantan

75123, Indonesia^2

Department of Aquaculture, Faculty of Fisheries and Marine Science, Diponegoro University, Indonesia^3

关键词: Aeromonas hydrophila;    Anti-bacterial activity;    Clear zones;    Colony forming units;    Inhibitory zones;    Oxytetracyclines (OTC);    Pathogenic bacterium;    Pseudomonas sp;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/144/1/012015/pdf
DOI  :  10.1088/1755-1315/144/1/012015

来源: IOP

【 摘 要 】

This study was conducted to evaluate the antibacterial activity of concoction Solanum ferox, Boesenbergia pandurata and Zingimber zerumbetextract (SF, BP, and ZZ) to inhibit pathogenic bacteria in tilapia with the each concentrations 600 ppm BP, 900 ppm SF and 200 ppm ZZ. Antibacterial activity was measured by testing the concoction of three plants extract against single isolate Aeromonas hydrophila and Pseudomonas sp. and combined both bacteria (105colony-forming units per milliliter). In this research, oxytetracycline was used as a control. Clear zone inhibition was observed at 6, 12, 18 and 24 hours after incubation at 30 °C. The results showed that the different concoction of BP: SF: ZZ have inhibitory zones against both single and joint isolate bacteria. The ratio of3:3:4 and 1:8:1 had higher antibacterial activity towards Pseudomonas sp. and 1:1:3 ratios both inhibit joint bacteria. The ZI% higher of concoction extracts against A.hydrophila is 1:1:8; 1:3:1; 3:4:3. The ZI% concoction extracts against Pseudomonas sp. ware 3:3:4 and 1:8:1 ratio. While the two bacteria combined, just 1:1:3 ratio had higher Z%. The conclusion is that a concoction of SF:BP:ZZ is effective to inhibit the growth of A.hydrophila and Pseudomonas sp., even its antibacterial ability is similar to the effectiveness of antibiotic oxytetracycline.