A laboratory-based study on patients with Parkinson’s disease and seborrheic dermatitis: the presence and density of Malassezia yeasts, their different species and enzymes production

Annals of Occupational and Environmental Medicine

Vladimir S Kostic¹  Jelena Marinkovic⁴  Berislav Vekic³  Aleksandra M Barac⁵  Danica Milobratovic²  Valentina S Arsic Arsenijevic⁵ 

[1]Institute of Neurology Clinical Centre of Serbia, University of Belgrade, School of Medicine, Belgrade, Serbia

[2]Department of Dermatology, Dermatology Unit, Clinical Center of Serbia

[3] Military Medical Centre, Belgrade, Serbia

[4]Management School, Alfa University, Belgrade, Serbia

[5]Institutes for Statistics and Medical Informatics, University of Belgrade School of Medicine, Belgrade, Serbia

[6]Institute of Microbiology and Immunology, University of Belgrade Medical School, Dr Subotica 1, Belgrade, Serbia

关键词: Enzymes;    Colony forming units;    Malassezia species;    Parkinson’s disease;    Seborrheic dermatitis;   

Others  :  814100
DOI  :  10.1186/1471-5945-14-5

 received in 2013-09-02, accepted in 2014-02-28,  发布年份 2014

【 摘 要 】

Background

Seborrheic dermatitis (SD) and Parkinson’s disease (PD) are frequently associated conditions. Aims of this study were: to determine severity of SD, presence of different species and density of Malassezia yeasts; to assess yeast lipases and phosphatases production in vitro and to compare these results between SD patients with and without PD.

Methods

This case–control prospective study was conducted at the Dermatology and Neurology Units, Clinical Centre of Serbia and at the National Medical Mycology Reference Laboratory, University of Belgrade Medical School, Serbia. A total of 90 patients and 70 healthy controls (HC) were investigated: 60 patients with SD (SDN) and 30 patients with SD and PD (SDP). Culture-based mycological examination was carried out on lesional skin (LS) and non-lesional skin (NLS). A yeasts density was determined by counting the Malassezia colony forming units per tape (CFU/tape). Enzymes production by isolated Malassezia was investigated.

Results

The most patients with SD were male (76.7%; SDP and 63.3%; SDN) and the intensity of SD was dominantly severe or moderate (76.7%; SDP and 75%; SDN). The presence of Malasseziа was high on LS in both groups (87.3%; SDP and 86.7%; SDN) (p=0.667).

The highest yeasts density (mean CFU/tape=67.8) was detected on LS in 53% of SDP group and in 21.7% of SDN group (mean CFU/tape=31.9) (p < 0.01). The presence of negative cultures was lower in SDP group (13.3%) in comparison to HC and SDN groups (37% and 31.7%, respectively). Malassezia density on NLS in SDP group (mean CFU/tape=44.3) was significantly higher in comparison to SDN and HC (p=0.018). M. globosa was the most abundant species identified amongst isolates from the SDP group (42.3%) and exhibited high production of phosphatase and lipase in vitro.

Conclusion

From this laboratory-based study a positive correlation between SD, PD, M. globosa incidence, high yeast density and high phosphatase and lipase activity was established. Our data lead to conclusion that local skin performance of PD patient’s characterized with increased sebum excretion ratio play a role in SD by stimulation of yeasts replication and enzyme production.

【 授权许可】

   
2014 Arsic Arsenijevic et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

【 参考文献 】

• [1]Yao SC, Hart AD, Terzella MJ: An evidence-based osteopathic approach to Parkinson disease. Osteopath Fam Physician 2013, 5:96-101.
• [2]de Lau LM, Breteler MM: Epidemiology of Parkinson’s disease. Lancet Neurol 2006, 5:525-535.
• [3]Kostić VS: Treatment of young-onset Parkinson’s disease: role of dopamine receptor agonists. Parkinsonism Relat Disord 2009, 15:71-75.
• [4]Shuster S, Thody AJ, Goolamali SK, Burton JL, Plummer N, Bates D: Melanocyte-stimulating hormone and parkinsonism. Lancet 1973, 3:463-464.
• [5]Binder RL, Jonelis FJ: Seborrheic dermatitis in neuroleptic induced Parkinsonism. Arch Dermatol 1983, 119:473-475.
• [6]Naldi L, Rebora A: Clinical practice: seborrheic dermatitis. N Engl J Med 2009, 360:387-396.
• [7]Marino CT, McDonald E, Romano JF: Seborrheic dermatitis in acquired immunodeficiency syndrome. Cutis 1991, 50:217-218.
• [8]Braak H, del Tredici K: Non-dopaminergic pathology in Parkinson’s disease. In The Non-Motor and Non-Dopaminergic Features of Parkinson’s Disease. Edited by Olanow CW, Stocchi F, Lang AE. Oxford: Blackwell Publishing Ltd; 2011:40-41.
• [9]Mastrolonardo M: Seborrheic dermatitis, increased sebum excretion and Parkinson’s disease: a survey of (im)possible links. Med Hypotheses 2003, 60:907-911.
• [10]Ashbee HR, Evans EGV: Immunology of disease associated with Malassezia species. Clin Microbiol Rev 2002, 15:21-57.
• [11]Gaitanis G, Magiatis P, Hantschke M, Bassukas ID, Velegraki A: The Malassezia genus in skin and systemic diseases. Clin Microbiol Rev 2012, 25:106-141.
• [12]Gupta AK, Batra R, Bluhm R, Boekhout T, Dawson TL Jr: Skin diseases associated with Malassezia species. J Am Acad Dermatol 2004, 51:785-798.
• [13]Gupta AK, Kohli Y, Summerbell RC, Faergemann J: Quantitative culture of Malassezia species from different body sites of individuals with or without dermatoses. Med Mycol 2001, 39:243-251.
• [14]Pechère M, Krischer J, Remondat C, Bertrand C, Trellu L, Saurat JH: Malassezia spp. carriage in patients with seborrheic dermatitis. J Dermatol 1999, 26:558-561.
• [15]Koca R, Altinyazar HC, Esturk E: Is topical metronidazole effective in seborrheic dermatitis? A double blind study. Int J Dermatol 2003, 42:632-635.
• [16]Pechère M, Remondat C, Bertrand C, Didierjean L, Saurat JH: A simple quantitative culture of Malassezia spp. in HIV-positive persons. Dermatology 1995, 191:348-349.
• [17]Guillot J, Guého E, Lesourd M, Midgley G, Chevrier G, Dupont B: Identification ofMalasseziaspecies. J Mycol Med 1996, 6:103-110.
• [18]Aubin F, Blanc D, Guinchard C, Agache P: Absence of minocycline in sebum? J Dermatol 1989, 16:369-373.
• [19]Villares JC, Carlini EA: Sebum secretion in idiopathic Parkinson’s disease: effect of anticholinergic and dopaminergic drugs. Acta Neurol Scand 1989, 80:57-63.
• [20]Gupta AK, Kohli Y: Prevalence of Malassezia species on various body sites in clinically healthy subjects representing different age groups. Med Mycol 2004, 42:35-42.
• [21]Wooten GF, Currie LJ, Bovbjerg VE, Lee JK, Patrie J: Are men at greater risk for Parkinson’s disease than women? J Neurol Neurosurg Psychiatry 2004, 75:637-639.
• [22]Hirtz D, Thurman DJ, Gwinn-Hardy K, Mohamed M, Chaudhuri AR, Zalutsky R: How common are the “common” neurologic disorders? Neurology 2007, 68:326-337.
• [23]Martignoni E, Godi L, Pacchetti C, Berardesca E, Vignoli GP, Albani G, Mancini F, Nappi G: Is seborrhea a sign of autonomic impairment in Parkinson’s disease? J Neural Transm 1997, 104:1295-1304.
• [24]Rincon S, Celis A, Sopo L, Motta A, Cepero de Garcia MC: Malassezia yeast species isolated from patients with dermatologic lesions. Biomedica 2005, 25:189-195.
• [25]Milani M, di Molfetta SA, Gramazio R, Fiorella C, Frisario C, Fuzio E, Marzocca V, Zurilli M, di Turi G, Felice G: Efficacy of betamethasone valerate 0.1% thermophobic foam in seborrhoeic dermatitis of the scalp: an open-label, multicentre, prospective trial on 180 patients. Curr Med Res Opin 2003, 19:342-345.
• [26]Bolognia JL: Dermatology. In Dermatology: 3e Expert Consult Premium Edition. Edited by Bolognia JL, Jorizzo JL, Schaffer JV. Spain: Elsevier; 2012:75-77.
• [27]Lee YW, Lee SY, Jung WH: Evaluation of expression of Lipases and Phospholipases of Malassezia in Patient with Seborrheic Dermatitis. Ann Dermatol 2013, 25:310-314.
• [28]Juntachai W, Oura T, Kajiwara S: Purification and characterization of a secretory lipolytic enzyme, MgLIP2, from Malassezia globosa. Microbiology 2011, 157:3492-3499.
• [29]Juntachai W, Oura T, Murayama SY, Kajiwara S: The lipolytic enzymes activities of Malassezia species. Med Mycology 2009, 47:477-484.
• [30]de Angelis YM, Gemmer CM, Kaczvinsky JR, Kenneally DC, Schwartz JR, Dawson TL Jr: Three etiologic facets of dandruff and seborrheic dermatitis: Malassezia fungi, sebaceous lipids and individual sensitivity. J Investig Dermatol Symp Proc 2005, 10:295-297.