The antibacterial activity of Scylla serrata haemolymph supernatant and Virgin Coconut Oil against the growth of Staphylococcus aureus ATCC 25923

MarSave International Symposium 2018: "Strengthening Marine Resilience for Sustainable Development Goals"

生态环境科学

Sudayasa, I.P.^1 ; Muin, M.^2 ; Kasih, Y.M.^2 ; Sulastrianah^3 ; Hafizah, I.^3

Departement of Public Health/Community Medicine, Medical Faculty, Halu Oleo University, Kendari, Indonesia^1

Medical Education Study Program, Medical Faculty, Halu Oleo University, Kendari, Indonesia^2

Integrated Laboratory, Medical Faculty, Halu Oleo University, Kendari, Indonesia^3

关键词: Anti-bacterial activity;    Antimicrobial peptide;    Experimental laboratory;    Infectious disease;    Inhibitory activity;    Minimum inhibition concentrations;    Staphylococcus aureus;    Virgin coconut oil;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/253/1/012019/pdf
DOI  :  10.1088/1755-1315/253/1/012019

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

There is a widespread increase in the number of cases of infectious diseases, including those caused by Staphylococcus aureus. Scylla serrata haemolymph contains active compounds, including antimicrobial peptide proteins as cofactor molecules in the antibody and immune system which resists infection. This research evaluated the antibacterial activity of Scylla serrata haemolymph supernatant and virgin coconut oil (VCO) against the growth of S. aureus ATCC 25923 strain. The research used an experimental laboratory design with post test control only and 3 replicates of each treatment. Samples of Scylla serrata haemolymph were centrifuged to extract the supernatant, which was divided into 9 treatment groups at different concentrations (100% to 0.39%). There were 2 control groups: positive control (Cefadroxil) and negative control (distilled water). Virgin coconut oil was produced using a traditional method for 9 hours and divided into 5 concentration groups (100% to 6.25%) with a positive control (Erythromycin 0.6%) and negative control (DMSO). The activity test used was inhibition zone diameter on nutrient agar and the minimum inhibition concentration (MIC) of each treatment was determined. The result showed that for Scylla serrata haemolymph supernatant the largest inhibition zone diameter was 16.83 mm with strong activity and the smallest was 5.58 mm with medium activity. The MIC of Scylla serrata haemolymph supernatant was 12.5%. For 100%, 50%, and 25% VCO the inhibition zone diameters were 2.67 mm, 0.91 mm, and 0.5 mm, respectively, with no inhibition at lower concentrations. We conclude that Scylla serrata haemolymph supernatant showed medium to strong inhibitory activity against the growth of S. aureus, while virgin coconut oil had weak activity and did not effectively inhibit the growth of S. aureus ATCC 25923.