Induction of synthesis of an antimicrobial peptide in the skin of the freeze‐tolerant frog, Rana sylvatica, in response to environmental stimuli

FEBS Letters

Matutte, Beverly¹  Knoop, Floyd C.²  Conlon, J.Michael¹  Storey, Kenneth B.³ 

[1] Regulatory Peptide Center, Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE 68178-0405, USA;Department of Medical Microbiology and Immunology, Creighton University Medical School, Omaha, NE 68178, USA;Institute of Biochemistry and Department of Biology, Carleton University, Ottawa, Ont., Canada K1S 5B6

关键词: Brevinin-1;    Antimicrobial peptide;    Amphibian skin;   

DOI  :  10.1016/S0014-5793(00)02102-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An extract of skin taken from specimens of the freeze-tolerant wood frog, Rana sylvatica, that were collected from cold (<7°C) ponds and maintained at 5°C lacked detectable antimicrobial activity. In contrast, an extract of skin taken from specimens maintained at 30°C for 3 weeks under laboratory conditions contained a high concentration (approximately 4 nmol/g) of a single antimicrobial peptide of the brevinin-1 family (FLPVVAGLAAKVLPSIICAVTKKC). The peptide inhibited growth of Escherichia coli (minimum inhibitory concentration 45 μM) and Staphylococcus aureus (minimum inhibitory concentration 7 μM). The data suggest that synthesis of the peptide is induced when the animal is in an environment that promotes the growth of microorganisms consistent with a role in the animal's defense strategy.

【 授权许可】

Unknown