Development of extract library from indonesian biodiversity: exploration of antibacterial activity of mangrove bruguiera cylindrica leaf extracts

3rd International Conference on Biological Sciences and Biotechnology

Audah, K.A.^1 ; Amsyir, J.^1 ; Almasyhur, F.^1 ; Hapsari, A.M.^1 ; Sutanto, H.^2

Department of Biomedical Engineering, Swiss German University, Prominence Tower, West Sutera Road No. 15, Alam Sutera, Tangerang, Banten

15143, Indonesia^1

Department of Chemical Engineering, Swiss German University, Prominence Tower, West Sutera Road No. 15, Alam Sutera, Tangerang, Banten

15143, Indonesia^2

关键词: Anti-bacterial activity;    antimicrobial;    Gram-positive bacterium;    Infectious disease;    Medicinal plants;    Natural products;    Staphylococcus aureus;    Staphylococcus epidermidis;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/130/1/012025/pdf
DOI  :  10.1088/1755-1315/130/1/012025

来源: IOP

【 摘 要 】

Antibacterial drugs derived from natural sources play significant roles in the prevention and treatment of bacterial infections since antibiotics have become less effective against many infectious diseases. Mangroves are very potential natural antibacterial sources among great numbers of wild medicinal plants. Bruguiera cylindrica is one of the many mangroves species which spread along Indonesian coastline. The aim of this study was to explore the antibacterial activity of B. cylindrica wet and dried leaf extracts. The wet extracts study was conducted with three different solvents system (water, ethanol, and n-Hexane) against Escherichia coli and Staphylococcus aureus. While, the dried extracts study was conducted with four different solvents system (water, ethanol, chloroform and n-Hexane) against three types of bacteria, Escherichia coli, Staphylococcus epidermidis and Staphylococcus aureus. The study showed that ethanol was the best solvent for extraction of phenolic and flavonoid. Antibacterial actitivity was measured by zone of inhibition which obtained from agar-disk diffusion method. The widest area of zone of inhibition was showed by wet extracts with ethanol against S. aureus and E. coli are 14.30 and 13.30 mm, respectively. While, the zone of inhibition dried extracts with ethanol against S. aureus, S. epidermidis and E. coli are 9.32, 6.59 and 6.20 mm, respectively. In conclusion, both type of extracts showed significant antibacterial activity against gram-positive bacteria as crude extracts.