Characterization of energetically functional inverted membrane vesicles from Corynebacterium glutamicum

FEBS Letters

Schrempp, S.¹  Bayan, N.¹  Shechter, E.¹ 

[1] Laboratoire des Biomembranes, Université de Paris Sud, 91405 Orsay Cedex, France

关键词: Gram-positive bacterium;    Membrane vesicle;    Membrane potential;    Protein translocation;    BHI;    brain heart infusion;    CCCP;    carbonyl cyanide m-chlorophenyl-hydrazone;    DCCD;    dicyclo-hexylcarbo-diimide;    PMS;    phenazine methosulfate;   

DOI  :  10.1016/0014-5793(94)01245-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We show that inverted membrane vesicles from Corynebacteriwn glutamicum, a Gram-positive bacterium, are able to generate and maintain an electrochemical gradient of protons in response to the addition of NADH. This result indicates that the respiratory chain is intact and that the vesicles are reasonably impermeable to protons. These membrane vesicles may be the starting point for in vitro translocation studies of proteins in Gram-positive bacteria.

【 授权许可】

Unknown