Protonmotive‐force‐driven leucine uptake in yeast plasma membrane vesicles

FEBS Letters

Konings, Wil N.²  Opekarová, Miroslava¹  Driessen, Arnold J.M.² 

[1] Department of General Microbiology, Institute of Microbiology, Czechoslovak Academy of Sciences, Videnská 1083, 142 20 Prague 4, Czechoslovakia;Department of Microbiology, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands

关键词: Membrane vesicle;    Leucine;    Amino acid transport;    (S. cerevisiae);   

DOI  :  10.1016/0014-5793(87)81462-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Plasma membranes prepared from Saccharomyces cerevisiae by the concanavalin A method were fused with liposomes by a freeze-thaw sonication method. A ΔΨ of −109 mV was generated in the sealed vesicles by a valinomycin-mediated potassium diffusion potential. This component of protonmotive force was able to drive a transient uptake of leucine.

【 授权许可】

Unknown