Leucine transport in plasma membrane vesicles of Saccharomyces cerevisiae

FEBS Letters

Opekarová, M.¹  Ramirez, J.²  Calahorra, M.²  Peña, A.² 

[1] Department of General Microbiology, Institute of Microbiology, Czechoslovak Academy of Sciences, Videnská 1083 142 20, Prague 4, Czechoslovakia;Department of Microbiology, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Apartado 70-600.04510, Mexico City, Mexico

关键词: Amino acid transport;    Leucine transport;    Electrochemical potential;    Proteoliposome;    Membrane vesicle;    (Yeast);    CCCP;    carbonylcyanide-m-chlorophenylhydrazone;    DiSC3(3);    dipropylthiacarbocyanine;    PC;    phosphatidylcholine;    PS;    phosphatidylserine;    TEA;    triethanolamine;    TMPD;    N;    N;    N′;    N′-tetramethyl-p-phenylenediamine;   

DOI  :  10.1016/0014-5793(89)81342-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Yeast plasma membrane vesicles were obtained by the fusion of liposomes with purified yeast membranes by means of the freeze thaw-sonication technique. Beef heart mitochondria cytochrome-c oxidase was incorporated into the vesicles. Addition of substrate (ascorbate/TMPD/cytochrome c) generated a membrane potential negative inside, and an alkaline pH gradient inside the vesicle, that served as the driving force for leucine transport. Both ΔpH and ΔΨ could drive leucine transport. When ΔpH was increased in the presence of valinomycin and potassium, at the expense of ΔΨ, leucine uptake increased by 10%.

【 授权许可】

Unknown