Evidence that stimulation of gluconeogenesis by fatty acid is mediated through thermodynamic mechanisms

FEBS Letters

Wallace, P.G.²  Phillips, J.W.²  Berry, M.N.²  Grivell, A.R.²  Henly, D.C.²  Welch, G.R.¹  Gregory, R.B.² 

[1] Department of Biological Sciences, University of New Orleans, Lakefront, New Orleans, LA 70148, USA;Department of Medical Biochemistry, School of Medicine, The Flinders University of South Australia, Bedford Park, SA 5042, Australia

关键词: Gluconeogenesis;    Metabolic regulation;    Linear force-flow relationship;    Irreversible thermodynamics;    Electrochemical potential;    Isolated hepatocyte;   

DOI  :  10.1016/0014-5793(88)80694-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have studied the stimulatory effects of palmitate on the rate of glucose synthesis from lactate in isolated hepatocytes. Control of the metabolic flow was achieved by modulating the activity of enolase using graded concentrations of fluoride. Unexpectedly, palmitate stimulated gluconeogenesis even when enolase was rate-limiting. This stimulation was also observed when the activities of phosphoenolpyruvate carboxykinase and aspartate aminotransferase were modulated using graded concentrations of quinolinate and aminooxyacetate, respectively. Linear force-flow relationships were found between the rate of gluconeogenesis and indicators of cellular energy status (i.e. mitochondrial membrane and redox potentials and cellular phosphorylation potential). These findings suggest that the fatty acid stimulation of glucose synthesis is in part mediated through thermodynamic mechanisms.

【 授权许可】

Unknown