Molecular cloning of a bombinin gene from Bombina orientalis: detection of NF‐κB and NF‐IL6 binding sites in its promoter

FEBS Letters

Barra, Donatella¹  Simmaco, Maurizio²  Miele, Rossella¹  Ponti, Donatella¹  Boman, Hans G³ 

[1] Istituto Pasteur-Fondazione Cenci Bolognetti, Dipartimento di Scienze Biochimiche `A. Rossi Fanelli' and CNR Centro di Biologia Molecolare, Università `La Sapienza', Piazzale Aldo Moro 5, 00185 Rome, Italy;Dipartimento di Scienze Biomediche, Università di Chieti, 66100 Chieti, Italy;Microbiology and Tumor Biology Center, Karolinska Institutet, Box 280, SE-171 77 Stockholm, Sweden

关键词: Antimicrobial peptide;    Glucocorticoid;    IκB;    NFκB;    NF-IL6;    Amphibia;    Bombina orientalis;    GC;    glucocorticoid;   

DOI  :  10.1016/S0014-5793(98)00718-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The sequence of a gene from Bombina orientalis was determined which codes for antibacterial peptides. The gene comprises two exons separated by a large intron. Exon 1 codes for the signal peptide, while exon 2 contains the genetic information for two identical bombinins and one bombinin H. The promoter region of the bombinin gene contains putative recognition sites for nuclear factors, such as NFκB and NF-IL6. In vivo experiments on B. orientalis have shown that a short contact with bacteria is sufficient to induce a marked increase in the amount of antibacterial peptides in the skin secretion of frogs. This increase was suppressed by pretreatment with glucocorticoids. In the latter case, a significant increase of IκBα in the secretion is also detectable.

【 授权许可】

Unknown