Antibacterial effectiveness of multi-strain probiotics supernatants intracanal medication on Enterococcus faecalis biofilm in a tooth model

BMC Oral Health

卷:23

Research

Gamal M. Hamad¹  Shymaa Shaaban²  Sybel Moussa²  Salma Genena²  Alaaeldin Elraggal³  Marwa A. Meheissen⁴ 

[1] Department of Food Technology, Arid Lands Cultivation Research Institute (ALCRI), City of Scientific Research and Technological Applications (SRTACity), New Borg El-Arab, Alexandria, Egypt;

[2] Division of Endodontics, Conservative Dentistry Department, Faculty of Dentistry, Alexandria University, Alexandria, Egypt;

[3] Division of Operative Dentistry, Conservative Dentistry Department, Faculty of Dentistry, Alexandria University, Alexandria, Egypt;

[4] Medical Microbiology & Immunology Department, Faculty of Medicine, Alexandria University, Alexandria, Egypt;

关键词: Probiotics;    Lactobacillus;    Intracanal medication;    Enterococcus faecalis;    Calcium hydroxide;   

DOI  :  10.1186/s12903-023-02914-2

 received in 2022-11-30, accepted in 2023-03-26,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundTo assess the antibacterial activity of multi-strain probiotics supernatants (MSP); Lactobacillus plantarum, Lactobacillus rhamnosus, and Lactobacillus acidophilus as an intracanal medication on Enterococcus faecalis (E. faecalis) biofilm in a tooth model.MethodsSixty extracted human single-rooted teeth with single canals were instrumented, sterilized, and inoculated with E. faecalis. After 21 days of incubation, four specimens were randomly selected to validate the biofilm formation by scanning electron microscope (SEM). The remaining specimens were randomly divided (n = 14), according to the intracanal medication (ICM) received into: Ca(OH)2: calcium hydroxide paste (35% Ultra Cal XS Ca(OH)2), Probiotics supernatants: MSP in poloxamer gel vehicle Poloxamer: poloxamer gel vehicle and, Control: E. faecalis biofilm only. The tested groups were further subdivided into two equal subgroups (n = 7) according to the incubation period (24 h and 7 days). Shaved dentin chips were obtained and collected by H-files and paper points, respectively for bacterial culture. The antibacterial activity was assessed after each incubation period quantitatively and qualitatively using bacterial colony-forming units per milliliter (CFUs/ml) and SEM, respectively.ResultsThe lowest CFUs/ml was found in Ca (OH)2 with a significant difference compared to other groups after 24 h. After 7 days, a similar outcome was found with a further significant reduction of CFUs/ml in all groups with no statistical difference between Ca(OH)2 and probiotics supernatants groups. Ca (OH)2 and Probiotics supernatants groups showed a significant (p < 0.05) percentage of overall bacterial reduction (100.00 ± 0.00% and 70.30 ± 12.95%, respectively) compared to poloxamer and control groups (27.80 ± 14.45 and 28.29 ± 19.79). SEM images showed a bacteria-free state in the Ca(OH)2 group after 7 days while few bacteria were found in the probiotics supernatants group. An extensive invasion of bacteria was found in poloxamer and controls groups.ConclusionMSP has a potential antibacterial effect on E. faecalis growth closely similar to the routinely used Ca (OH)2.

【 授权许可】

CC BY   
© The Author(s) 2023

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