Characterizing the Catalytic Potential of Deinococcus, Arthrobacter and other Robust Bacteria in Contaminated Subsurface Environments of the Hanford Site

Fredrickson, Jim K. ; Daly, Michael J.

Pacific Northwest National Laboratory (U.S.)

关键词: 12 Management Of Radioactive Wastes, And Non-Radioactive Wastes From Nuclear Facilities;    Lactobacillus;    Ionizing Radiations;    59 Basic Biological Sciences;    Sensitivity;   

DOI  :  10.2172/895911
RP-ID  :  ERSD-1024843-2006
RP-ID  :  None
RP-ID  :  895911

美国|英语

来源: UNT Digital Library

【 摘 要 】

Until recently, there have been no clear physiologic predictors of a cell's ability to recover from ionizing radiation (IR), desiccation, and other DOE-relevant oxidative stress conditions. In general, the most resistant bacteria have been Gram-positive (e.g., Deinococcus, Arthrobacter, Lactobacillus & Enterococcus spp.) and the most sensitive have been Gram-negative (e.g., Pseudomonas, Shewanella & Neisseria spp.). However, there are several reported exceptions to this paradigm, the Gram-negative cyanobacterium Chroococcidiopsis is extremely resistant to IR, whereas the Gram-positive Micrococcus luteus is sensitive. We have identified biomolecular signatures for radiation sensitivity and resistance which are independent of phylogeny, where very high and very low intracellular Mn/Fe concentration ratios correlated with very high and very low resistances, respectively; and restricting Mn(II) in the famously resistant Deinococcus radiodurans sensitized this eubacterium to IR (http://cfyn.ifas.ufl.edu/radiation.pdf).