Assessment of cecal microbiota modulation from piglet dietary supplementation with copper

BMC Microbiology

article

Paganin, Ana Carolina Laureano¹  Monzani, Paulo Sérgio²  Carazzolle, Marcelo Falsarella³  Araujo, Raquel Bighetti⁴  Gonzalez-Esquerra, Ricardo⁴  Haese, Douglas⁵  Kill, João L⁵  Rezende, Graziela Silva¹  de Lima, César Gonçalves²  Malavazi, Iran¹  de Melo Freire, Caio César¹  da Cunha, Anderson Ferreira¹ 

[1] Departamento de Genética e Evolução, Universidade Federal de São Carlos;Faculdade de Zootecnia e Engenharia de Alimentos, Departamento de Medicina Veterinária, Universidade de São Paulo;Departamento de Genética, Evolução, Microbiologia e Imunologia, Universidade de Campinas;Novus International;Centro de Tecnologia Animal Ltda

关键词: 16S rRNA gene;    Microbiota;    Swine;    Copper;    Feed supplement;    Animal nutrition;   

DOI  :  10.1186/s12866-023-02826-9

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

Swine production expanded in the last decades. Efforts have been made to improve meat production and to understand its relationship to pig gut microbiota. Copper (Cu) is a usual supplement to growth performance in animal production. Here, two performance studies were conducted to investigate the effects of three different sources of Cu on the microbiota of piglets. A total of 256 weaned piglets were randomly allocated into 4 treatments (10 replicates per treatment of 4 piglets per pen in Trial 1 and 8 replicates of 3 piglets per pen in Trial 2). Treatments included a control group (fed 10 mg/kg of Cu from CuSO4), a group fed at 160 mg/kg of Copper (II) sulfate (CuSO4) or tri-basic copper chloride (TBCC), and a group fed with Cu methionine hydroxy analogue chelated (Cu-MHAC) at 150, 80, and 50 mg/kg in Phases 1 (24–35 d), 2 (36–49 d), and 3 (50–70 d), respectively. At 70 d, the cecum luminal contents from one pig per pen were collected and polled for 16 S rRNA sequencing (V3/V4 regions). Parameters were analyzed in a completely randomized block design, in which each experiment was considered as a block. A total of 1337 Operational Taxonomic Units (OTUs) were identified. Dominance and Simpson ecological metrics were statistically different between control and treated groups (P < 0.10) showing that different Cu sources altered the gut microbiota composition with the proliferation of some bacteria that improve gut health. A high abundance of Prevotella was observed in all treatments while other genera were enriched and differentially modulated, according to the Cu source and dosage. The supplementation with Cu-MHAC can modify a group of bacteria involved in feed efficiency (FE) and short chain fatty acids (SCFA) production (Clostridium XIVa, Desulfovibrio, and Megasphera). These bacteria are also important players in the activation of ghrelin and growth hormones that were previously reported to correlate with Cu-MHAC supplementation. These results indicated that some genera seem to be directly affected by the Cu source offered to the animals. TBCC and Cu-MHAC (even in low doses) can promote healthy modifications in the gut bacterial composition, being a promising source of supplementation for piglets.

【 授权许可】

CC BY|CC0