Similarity of salivary microbiome in parents and adult children

PeerJ

article

Kati Sundström¹  Pashupati P. Mishra²  Mikko J. Pyysalo⁵  Terho Lehtimäki²  Pekka J. Karhunen¹  Tanja Pessi¹ 

[1] Department of Forensic Medicine, Faculty of Medicine and Life Sciences, University of Tampere;Department of Clinical Chemistry, Faculty of Medicine and Health Technology, Tampere University;Finnish Cardiovascular Research Center-Tampere, Faculty of Medicine and Health Technology, Tampere University;Department of Clinical Chemistry, Fimlab Laboratories Ltd;Department of Otorhinolaryngology, Faculty of Medicine and Health Technology, Tampere University;Department of Oral and Maxillofacial Diseases, Tampere University Hospital;Oral Health Services;Department of Molecule Microbiology, Faculty of Medicine and Health Technology, Tampere University;Science Centre

关键词: Metagenomics;    Next generation sequencing;    Saliva;    Microbiota;    Similarity;    16S rRNA gene;   

DOI  :  10.7717/peerj.8799

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Background Human saliva contains approximately 700 bacterial species. It has been reported that the salivary microbiome of a large family of closely related individuals consisting of multiple households is similar but the relatedness of salivary bacteria between generations of parents and their children has not yet been investigated. The objectives were to investigate the entirety of salivary bacterial DNA profiles and whether and how families share these profiles and also compare these communities between grandparents and their first daughter generations (F1) using 16S rRNA gene amplicon sequencing. Results The most abundant phyla in two separate families were Firmicutes, Bacteroidetes, Proteobacteria, Fusobacteria and Actinobacteria. Family ties explained 13% of the variance between individuals’ bacterial communities (R2 = 0.13; P = 0.001). Mothers shared more OTUs with adult children compared to fathers, but this linkage seemed to be weaker in the nuclear family with older adult children. We identified 29 differentially abundant genus level OTUs (FDR < 0.05) between families, which accounted for 31% of the total identified genus level OTUs. Conclusions Our results indicate that adult family members share bacterial communities and adult children were more similar to mothers than fathers. The observed similarity in oral microbiome between parent–child pairs seemed to weaken over time. We suggest that our analysis approach is suitable for relatedness study of multigenerational salivary bacteria microbiome.

【 授权许可】

CC BY