Detection and analysis of hemolysin genes in Aeromonas hydrophila isolated from Gouramy (Osphronemus gouramy) by polymerase chain reaction (PCR)

Asean-Fen International Fisheries Symposium - 2017

Rozi^1 ; Rahayu, K.^1 ; Daruti, D.N.^1

Department of Fish Health Management and Aquaculture, Faculty of Fisheries and Marine, Airlangga University, Surabaya

60115, Indonesia^1

关键词: 16S rRNA gene;    Aeromonas hydrophila;    DNA sequencing analysis;    Gel electrophoresis;    Gene sequences;    Haemolysins;    Representative strain;    Species specifics;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/137/1/012001/pdf
DOI  :  10.1088/1755-1315/137/1/012001

来源: IOP

【 摘 要 】

The goal of this study was to detect of Aeromonas hydrophila carrying the hlyA gene in guramy by PCR assay. A total of 5 A. hydrophila strains were isolated from gouramy with different location and furthermore genotypic of all A. hydrophila strains havedetected by PCR assay for 16S rRNA gene. The primers used in the PCR targeted a 592-bp fragment of the hlyA gene coding for the hemolysin gene. Particularly hlyA genes are responsible for haemolysin toxins production in this genus. After gel electrophoresis, the amplicons from representative strains of the A. hydrophila were purified using extraction kit and were subjected to the DNA sequencing analysis. The results showed that: (i) the 592bp amplicon of the hlyA gene was detected in 5/6 of the A. hydrophila; (ii) the nucleotide blast results of hemolysin gene sequences of the strains of A. hydrophila revealed a high homology of 90-97 % with published sequences, and;(iii) the protein blast showed 95-98 % homology when compared to the published sequences. The PCR clearly identified the haemolysin-producing strains of A. hydrophila by detection in hlyA genes and may have application as a rapid species-specific virulence test.