Saponin Isolation as Main Ingredients of Insecticide and Collagen Type I From Crown of Thorn_Starfish (Acanthaster planci)

2nd International Conference on Applied Marine Science and Fisheries Technology 2017

地球科学;农业科学

Wijanarko, Anondho^1 ; Januardi Ginting, Mikael^2 ; Sahlan, Muhamad^1 ; Krisanta Endah Savitri, Imelda^3 ; Florensia, Yunita^1 ; Regina Sudiarta, Maria^1 ; Pastika, Satria^1 ; Rafiki, Fakhri^1 ; Hermansyah, Heri^1

Department of Chemical Engineering, Universitas Indonesia, Kampus Baru UI Depok, 16424, Indonesia^1

Department of Biology, Universitas Indonesia, Kampus Baru UI Depok, 16424, Indonesia^2

Department of Aquatic Product Technology, Faculty of Fishery, Pattimura University, Kampus Unpatti-Poka, Ambon

97233, Indonesia^3

关键词: Amino acid compositions;    Cytotoxic compounds;    Environment friendly;    Enzymatic extraction;    Extraction solvents;    Gel electrophoresis;    Multi-stage extraction;    Quantitative and qualitative analysis;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/89/1/012032/pdf
DOI  :  10.1088/1755-1315/89/1/012032

学科分类：农业科学（综合）

来源: IOP

【 摘 要 】

The outbreaks of crown of thorns starfish (Acanthaster planci) resulted in the severe destruction of coral reefs in a large number of Indonesia's marine ecosystem, especially in the western part. At the moment, control efforts are proven to be ineffective because of its high cost and labor intensive. Recent research found that A. planci contain saponins that act as cytotoxic compound and can be used as an environment-friendly insecticide to eradicate Kalotermitidae pest. Saponins extracted by maceration using ethanol 96.0% with a total yield of saponins 9.04% and 4.66% for two test. Purification of saponin was achieved by utilization of activated carbon with a mass of carbon:volume sample 1:2 (w/v) and stirred for 20 minutes. Sapogenin can be isolated by hydrolyzing using hydrochloric acid, and thus 168.4 mg sapogenin is obtained. In addition to saponins, A. planci also contains collagen Type I. Collagen isolation by multistage extraction began with extracting the collagen with alkaline solvent, with water, NaOH 0.1 M, and Ca(OH)2 0.2 M as the solvent variations. The second step is acid-enzymatic extraction by pepsin digestion in 0.5 M acetic acid. Collagen extract will be further purified by salting out and dialysis method to obtain pure collagen yield called Pepsin Solubilized Collagens (PSC). Characterization of PSC consists of quantitative and qualitative analysis such as Lowry method, gel electrophoresis, UV spectroscopy, amino acid composition analysis, and Scanning Electron Microscopy (SEM). The result shows Ca(OH)20.2 M as the best extraction solvent with 2.26% yield of PSC.