2ab assembly: a methodology for automatable, high-throughput assembly of standard biological parts

Journal of Biological Engineering

关键词: 2ab reaction;    Automated assembly;    DNA fabrication;    Synthetic biology;   

DOI  :  10.1186/1754-1611-7-2

来源: DOAJ

【 摘 要 】

Abstract

There is growing demand for robust DNA assembly strategies to quickly and accurately fabricate genetic circuits for synthetic biology. One application of this technology is reconstitution of multi-gene assemblies. Here, we integrate a new software tool chain with 2ab assembly and show that it is robust enough to generate 528 distinct composite parts with an error-free success rate of 96%. Finally, we discuss our findings in the context of its implications for biosafety and biosecurity.

【 授权许可】

Unknown