2ab assembly: a methodology for automatable, high-throughput assembly of standard biological parts

Journal of Biological Engineering

J Christopher Anderson³  Angel Asante²  Douglas Densmore¹  Jennifer AN Brophy²  Mariana Leguia³ 

[1] Current address: Department of Electrical and Computer Engineering, Boston University, Boston, MA, 02215, USA;Department of Bioengineering, University of California, Berkeley, CA, 94720, USA;Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA

关键词: Synthetic biology;    DNA fabrication;    Automated assembly;    2ab reaction;   

Others  :  806025
DOI  :  10.1186/1754-1611-7-2

 received in 2012-07-24, accepted in 2013-01-01,  发布年份 2013

【 摘 要 】

There is growing demand for robust DNA assembly strategies to quickly and accurately fabricate genetic circuits for synthetic biology. One application of this technology is reconstitution of multi-gene assemblies. Here, we integrate a new software tool chain with 2ab assembly and show that it is robust enough to generate 528 distinct composite parts with an error-free success rate of 96%. Finally, we discuss our findings in the context of its implications for biosafety and biosecurity.

【 授权许可】

   
2013 Leguia et al.; licensee BioMed Central Ltd.

【 图 表 】

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