Core Fucosylation of Maternal Milk N-Glycan Evokes B Cell Activation by Selectively Promoting the l-Fucose Metabolism of Gut Bifidobacterium spp. and Lactobacillus spp

mBio

Zhongfu Wang¹  Patrice Malard²  Wei Huang³  Yang Liu⁴  Di Mei⁴  Ming Li⁵  Yaqiang Bai⁵  Jieli Yuan⁵  Jiaorui Zhou⁵  Qingjie Fan⁵  Qiulong Yan⁵  Jingyu Yan⁶  Wenzhe Li⁷  Jia Tao⁸  Naoyuki Tanigchi⁹  Jianguo Gu^1,10 

[1] Care (BINC), Guangzhou, China;;Biostime Institute Nutrition &CAS Key Laboratory of Receptor Research, Chinese Academy of Sciences, Shanghai Institute of Materia Medica, Shanghai, China;Clinical Laboratory of Huludao Center Hospital, Huludao, China;College of Basic Medical Science, Dalian Medical University, Dalian, China;Dalian Institute of Chemical Physics, Key Laboratory of Separation Science for Analytical Chemistry, Chinese Academy of Sciences, Dalian, China;Department of Glyco-Oncology, Osaka International Cancer Institute, Osaka, Japan;Department of Gynaecology, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China;Division of Regulatory Glycobiology, Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University, Sendai, Japan;Educational Ministry Key Laboratory of Resource Biology and Biotechnology in Western China, Life Science College, Northwest University, Xian, China;

关键词: B cells;    Bifidobacterium;    Lactobacillus;    core fucosylation;    infants;    milk N-glycan;   

DOI  :  10.1128/mBio.00128-19

来源: DOAJ

【 摘 要 】

ABSTRACT The maternal milk glycobiome is crucial for shaping the gut microbiota of infants. Although high core fucosylation catalyzed by fucosyltransferase 8 (Fut8) is a general feature of human milk glycoproteins, its role in the formation of a healthy microbiota has not been evaluated. In this study, we found that the core-fucosylated N-glycans in milk of Chinese mothers selectively promoted the colonization of specific gut microbial groups, such as Bifidobacterium spp. and Lactobacillus spp. in their breast-fed infants during lactation. Compared with Fut8+/+ (WT) mouse-fed neonates, the offspring fed by Fut8+/− maternal mice had a distinct gut microbial profile, which was featured by a significant reduction of Lactobacillus spp., Bacteroides spp., and Bifidobacterium spp. and increased abundance of members of the Lachnospiraceae NK4A136 group and Akkermansia spp. Moreover, these offspring mice showed a lower proportion of splenic CD19+ CD69+ B lymphocytes and attenuated humoral immune responses upon ovalbumin (OVA) immunization. In vitro studies demonstrated that the chemically synthesized core-fucosylated oligosaccharides possessed the ability to promote the growth of tested Bifidobacterium and Lactobacillus strains in minimal medium. The resulting L-fucose metabolites, lactate and 1,2-propanediol, could promote the activation of B cells via the B cell receptor (BCR)-mediated signaling pathway. IMPORTANCE This study provides novel evidence for the critical role of maternal milk protein glycosylation in shaping early-life gut microbiota and promoting B cell activation of neonates. The special core-fucosylated oligosaccharides might be promising prebiotics for the personalized nutrition of infants.

【 授权许可】

Unknown