Fabrication of 6-gingerol, doxorubicin and alginate hydroxyapatite into a bio-compatible formulation: enhanced anti-proliferative effect on breast and liver cancer cells

Chemistry Central Journal

Dulharie T. Wijeratne¹  Gathsaurie Neelika Malavige¹  Rohini M. de Silva²  Danushika C. Manatunga²  K. M. Nalin de Silva²  Gareth Williams³ 

[1] Centre for Dengue Research, Department of Microbiology, Faculty of Medical Sciences, University of Sri Jayewardenepura;Department of Chemistry, University of Colombo;UCL School of Pharmacy, University College London;

关键词: Hydroxyapatite;    6-Gingerol;    Doxorubicin;    MCF-7;    HEpG2;   

DOI  :  10.1186/s13065-018-0482-6

来源: DOAJ

【 摘 要 】

Abstract Ample attention has been devoted to the construction of anti-cancer drug delivery systems with increased stability, and controlled and targeted delivery, minimizing toxic effects. In this study we have designed a magnetically attractive hydroxyapatite (m-HAP) based alginate polymer bound nanocarrier to perform targeted, controlled and pH sensitive drug release of 6-gingerol, doxorubicin, and their combination, preferably at low pH environments (pH 5.3). They have exhibited higher encapsulation efficiency which is in the range of 97.4–98.9% for both 6-gingerol and doxorubicin molecules whereas the co-loading has accounted for a value of 81.87 ± 0.32%. Cell proliferation assays, fluorescence imaging and flow cytometric analysis, demonstrated the remarkable time and dose responsive anti-proliferative effect of drug loaded nanoparticles on MCF-7 cells and HEpG2 cells compared with their neat counter parts. Also, these systems have exhibited significantly reduced toxic effects on non-targeted, non-cancerous cells in contrast to the excellent ability to selectively kill cancerous cells. This study has suggested that this HAP based system is a versatile carrier capable of loading various drug molecules, ultimately producing a profound anti-proliferative effect.

【 授权许可】

Unknown