Magnetic nanoparticles sensitize MCF-7 breast cancer cells to doxorubicin-induced apoptosis

World Journal of Surgical Oncology

Fawzi M Alsheyab³  Khaled Aledealat³  BA Albiss¹  Ahmad N Aldaher²  M-Ali H Al-Akhras³  Nizar M Mhaidat²  Khaled Aljarrah³ 

[1] Magnetic measurements laboratory, Jordan University of Science and Technology, Irbid, Jordan;Faculty of Pharmacy, University of Science and Technology, Irbid, Jordan;Biophysics laboratory, Jordan University of Science and Technology, Irbid, Jordan

关键词: Breast cancer;    Doxorubicin;    MCF-7;    Nanoparticles;   

Others  :  827870
DOI  :  10.1186/1477-7819-10-62

 received in 2011-05-30, accepted in 2012-04-25,  发布年份 2012

【 摘 要 】

Background

Resistance of breast cancer cells to the available chemotherapeutics is a major obstacle to successful treatment. Recent studies have shown that magnetic nanoparticles might have significant application in different medical fields including cancer treatment. The goal of this study is to verify the ability of magnetic nanoparticles to sensitize cancer cells to the clinically available chemotherapy.

Methods

The role of iron oxide nanoparticles, static magnetic field, or a combination in the enhancement of the apoptotic potential of doxorubicin against the resistant breast cancer cells, MCF-7 was evaluated using the MTT assay and the propidium iodide method.

Results

In the present study, results revealed that pre-incubation of MCF-7 cells with iron oxide nanoparticles before the addition of doxorubicin did not enhance doxorubicin-induced growth inhibition. Pre-incubation of MCF-7 cells with iron oxide nanoparticles followed by a static magnetic field exposure significantly (P < 0.05) increased doxorubicin-induced cytotoxicity. Sensitization with pre-exposure to the magnetic field was dose-dependent where the highest cytotoxicity was seen at 1 tesla. Further experiments revealed that the anti-proliferative effect of this treatment procedure is due to induction of apoptotic cell death.

Conclusions

These results might point to the importance of combining magnetic nanoparticles with a static magnetic field in treatment of doxorubicin-refractory breast cancer cells.

【 授权许可】

   
2012 Aljarrah et al.; licensee BioMed Central Ltd.

【 图 表 】

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