Comparison of accelerated and enzyme-associated real-time degradation of HMW PLLA and HMW P3HB films

Polymer Testing

Kerstin Lebahn¹  Stefan Oschatz²  Niels Grabow³  Thomas Reske³  Daniela Arbeiter³  Klaus-Peter Schmitz³  Thomas Eickner³  Volkmar Senz⁴ 

[1] Corresponding author.;Institute for Implant Technology and Biomaterials e.V, Friedrich-Barnewitz-Straße 4, 18119, Rostock-Warnemünde, Germany;Institute for Biomedical Engineering, Rostock University Medical Center, Friedrich-Barnewitz-Straße 4, 18119, Rostock-Warnemünde, Germany;Institute for Implant Technology and Biomaterials e.V, Friedrich-Barnewitz-Straße 4, 18119, Rostock-Warnemünde, Germany;

关键词: Poly-l-lactide;    Poly-3-hydroxybutyrate;    Enzyme-associated degradation;    Accelerated degradation;    Molecular weight;    Crystallinity;   

DOI  :  

来源: DOAJ

【 摘 要 】

Designing laboratory-scale degradation experiments for polymer-based biomaterials is crucial for the development of safe and functional implants, in particular regarding high molecular weight polyesters. Within this work, we compared accelerated degradation of solvent cast HMW-PLLA and HMW-P3HB films at 55 °C (16 weeks) with enzyme-associated (proteinase K and lipases) real-time degradation at 37 °C (108 weeks). During real-time degradation, PLLA showed mass loss up to 83%, in contrast to accelerated conditions, where no changes occurred. Moreover, we observed wave-shape development of crystallinity for PLLA and PHB for both degradation conditions applied, whereby PLLA χ value nearly doubled to up to 75%. These results were used to develop a correlation model based on molecular weight decrease and were furthermore discussed in light of a detailed literature review. In summary, real-time in vitro studies could be adapted to accelerated protocols providing the same limiting conditions such as molecular weight and initial crystallinity are given.

【 授权许可】

Unknown