Mixed Cultures Fermentation for the Production of Poly-ß-hydroxybutyrate

Brazilian Archives of Biology and Technology

Thomas Shalin¹  Raveendran Sindhu¹  Parameswaran Binod¹  Carlos Ricardo Soccol¹  Ashok Pandey¹ 

关键词: Poly-3-hydroxybutyrate;    Biopolymer;    Mixed culture;    Bacillus firmus;    Lactobacillus delbrueckii;    Biodegradation;   

DOI  :  10.1590/S1516-89132013005000016

来源: SciELO

【 摘 要 】

Poly-ß-hydroxybutyrate (PHB) is a biodegradable intracellular microbial product produced by many bacteria and it is comparable to some of the petrochemical derived thermoplastics such as polypropylene. One of the main barriers for the commercial exploitation is the high cost of the substrate for the production of biopolymer. The utilization of mixed microbial cultures facilitates the use of complex substrates thereby reducing the cost of PHB production. In the present study, mixed culture systems were evaluated for PHB production. Bacillus firmus NII 0830 was used for the production of PHB since it accumulates a large amount of PHB and a second organism Lactobacillus delbrueckii NII 0925 was used to provide lactic acid. FTIR and 1H NMR analyses revealed that the PHB extracted from pure culture and mixed culture showed exact match to that of standard PHB. Biodegradation studies of the PHB blends showed 87% degradation. It was also found that a consortium of organisms degraded the films faster than a single organism.

【 授权许可】

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