Insights into the structure of Escherichia coli outer membrane as the target for engineering microbial cell factories

Microbial Cell Factories

Jianli Wang¹  Xiaoyuan Wang²  Wenjian Ma³ 

[1] State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, 214122, Wuxi, China;International Joint Laboratory On Food Safety, Jiangnan University, 214122, Wuxi, China;State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, 214122, Wuxi, China;Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, China;State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, 214122, Wuxi, China;Science Center for Future Foods, Jiangnan University, 214122, Wuxi, China;

关键词: Outer membrane;    Lipopolysaccharide;    Exopolysaccharide;    Flagella;    Fimbria;    Membrane engineering;    Poly-3-hydroxybutyrate;    Inclusion bodies;    Microbial cell factories;    Escherichia coli;   

DOI  :  10.1186/s12934-021-01565-8

来源: Springer

【 摘 要 】

Escherichia coli is generally used as model bacteria to define microbial cell factories for many products and to investigate regulation mechanisms. E. coli exhibits phospholipids, lipopolysaccharides, colanic acid, flagella and type I fimbriae on the outer membrane which is a self-protective barrier and closely related to cellular morphology, growth, phenotypes and stress adaptation. However, these outer membrane associated molecules could also lead to potential contamination and insecurity for fermentation products and consume lots of nutrients and energy sources. Therefore, understanding critical insights of these membrane associated molecules is necessary for building better microbial producers. Here the biosynthesis, function, influences, and current membrane engineering applications of these outer membrane associated molecules were reviewed from the perspective of synthetic biology, and the potential and effective engineering strategies on the outer membrane to improve fermentation features for microbial cell factories were suggested.

【 授权许可】

CC BY