Microbial production and characterization of poly-3-hydroxybutyrate by Neptunomonas antarctica

PeerJ

article

Xiao-Jie Liu¹  Jie Zhang¹  Peng-Hui Hong¹  Zheng-Jun Li¹ 

[1] Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology

关键词: Halophilic;    Neptunomonas antarctica;    Poly-3-hydroxybutyrate;    Seawater;    Marine bacteria;   

DOI  :  10.7717/peerj.2291

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Considering the industrial interest of biodegradable polymer poly-3-hydroxybutyrate (PHB), the marine bacteria Neptunomonas antarctica was studied for its ability to accumulate PHB. The extracted polymer was confirmed to be PHB by nuclear magnetic resonance analysis. In shake flask cultures using natural seawater as medium components, PHB was produced up to 2.12 g/L with a yield of 0.18 g PHB/g fructose. In the presence of artificial seawater, the PHB titer and yield reached 2.13 g/L and 0.13 g PHB/g fructose, respectively. The accumulated polymer gradually decreased when fructose was exhausted, indicating that intracellular PHB was degraded by N. antarctica. The weight-average and number-average molecular weights of PHB produced within natural seawater were 2.4 × 105 g/mol and 1.7 × 105 g/mol, respectively. Our results highlight the potential of N. antarctica for PHB production with seawater as a nutrient source.

【 授权许可】

CC BY