Quantification of ruffle area and dynamics in live or fixed lung adenocarcinoma cells

STAR Protocols

Mariana Cooke¹  Marcelo G. Kazanietz²  Gabriel Kreider-Letterman³  Rafael Garcia-Mata³  Silvia M. Goicoechea⁴ 

[1] Corresponding author;Department of Medicine, Einstein Medical Center Philadelphia, Philadelphia, PA 19141, USA;Department of Biological Sciences, University of Toledo, Toledo, OH 43606, USA;Department of Systems Pharmacology and Translational Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA;

关键词: Cancer;    Cell Biology;    Cell culture;    Cell-based Assays;    Microscopy;    Single Cell;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Ruffles are actin-rich membrane protrusions implicated in actin reorganization and initiation of cell motility. Here, we describe methods for measuring and analyzing ruffle dynamics in live cells and average ruffle area per cell in fixed samples. The specific steps described are for the analysis of A549 lung adenocarcinoma cells, but the protocol can be applied to other cell types. The protocol has applications for dissecting the signaling events linked to ruffling.For complete details on the use and execution of this protocol, please refer to Cooke et al. (2021). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

【 授权许可】

Unknown