Intestinal organoid co-culture protocol to study cell competition in vitro

STAR Protocols

Milou S. van Driel¹  Louis Vermeulen²  Rana Ramadan²  Sanne M. van Neerven³  David J. Huels³ 

[1] Corresponding author;Oncode Institute, Meibergdreef 9, 1105 Amsterdam, the Netherlands;Laboratory for Experimental Oncology and Radiobiology, Center for Experimental and Molecular Medicine, Cancer Center Amsterdam and Amsterdam Gastroenterology Endocrinology and Metabolism, Amsterdam University Medical Centers, Meibergdreef 9, 1105 Amsterdam, the Netherlands;

关键词: Cell Biology;    Cell culture;    Flow Cytometry/Mass Cytometry;    Cell-based Assays;    Cancer;    Microscopy;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Intestinal organoid cultures are a powerful tool to study epithelial cells in vitro, as they are able to proliferate and differentiate into all cell lineages observed in vivo. Co-culturing organoids with distinct genetic backgrounds provides an excellent approach to study contact dependent and independent interactions between healthy and mutant epithelial intestinal cells. Here, we provide 2D and 3D approaches to mouse organoid co-cultures using fluorescently labeled organoids and demonstrate the analysis of these co-cultures using flow cytometry and microscopy-based approaches.For complete details on the use and execution of this profile, please refer to van Neerven et al., 2021.

【 授权许可】

Unknown