Protocols for analysis of mitochondrial permeability transition pore opening in mouse somatic cell reprogramming

STAR Protocols

卷:2

Yanmin Xin¹  Zihuang Liu¹  Xingguo Liu¹  Junwei Wang²  Zhongfu Ying²  Ge Xiang² 

[1] Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, CUHK-GIBH Joint Research Laboratory on Stem Cells and Regenerative Medicine, Institute for Stem Cell and Regeneration, Guangzhou Institutes of Biomedicine and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Guangzhou 510530, China;

[2] CAS Key Laboratory of Regenerative Biology, Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou Medical University, Guangzhou 510530, China;

关键词: Cell Biology;    Cell-based Assays;    Flow Cytometry/Mass Cytometry;    Metabolism;    Microscopy;    Stem Cells;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) by defined factors. Here, we describe a protocol for imaging mitochondrial permeability transition pore (mPTP) opening in reprogramming of somatic cells using a confocal microscope. We also describe a method to sort high and low mPTP opening somatic cells by calcein fluorescence and reprogram these sorted cells to iPSCs. These protocols are also suitable for imaging mPTP opening and uncovering the mechanisms of mPTP function in other cell fate conversions.For complete details on the use and execution of this protocol, please refer to Ying et al. (2018).

【 授权许可】

Unknown