FACS-based protocol to assess cytotoxicity and clonogenic potential of colorectal cancer stem cells using a Wnt/β-catenin signaling pathway reporter

STAR Protocols

Miriam Gaggianesi¹  Simone Di Franco²  Jan Paul Medema²  Melania Lo Iacono²  Giorgio Stassi³  Le Zhang³ 

[1] Oncode Institute, Amsterdam UMC, University of Amsterdam, Amsterdam 1105 AZ, the Netherlands;Department of Surgical Oncological and Stomatological Sciences, University of Palermo, Palermo 90127, Italy;Laboratory for Experimental Oncology and Radiobiology, Center for Experimental and Molecular Medicine, Cancer Center Amsterdam, Amsterdam UMC, University of Amsterdam, Amsterdam 1105 AZ, the Netherlands;

关键词: Cell Biology;    Cell isolation;    Flow Cytometry/Mass Cytometry;    Cell-based Assays;    Cancer;    Signal Transduction;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Cancer stem cells (CSCs) play a key role in tumor initiation and progression. A real-time tool to evaluate the activation of CSC-specific signaling pathways is crucial for the study of this cancer cell subset. Here, we present a protocol to monitor, in vitro, the activation of Wnt/β-catenin signaling pathway, which is considered a functional biomarker for colorectal CSCs (CR-CSCs). This flow-cytometry-based protocol allows it to isolate CR-CSCs and to evaluate their cytotoxicity upon anti-tumor treatments.For complete details on the use and execution of this protocol, please refer to Di Franco et al. (2021).

【 授权许可】

Unknown