Generation of effector Vγ9Vδ2 T cells and evaluation of their response to phosphoantigen-loaded cells

STAR Protocols

Andrew J. Wiemer¹  Chia-Hung Christine Hsiao² 

[1]Corresponding author

[2]Department of Pharmaceutical Sciences, University of Connecticut, Storrs, CT 06269, USA

关键词: Cell Biology;    Cell culture;    Cell isolation;    Cell-based Assays;    Cell separation/fractionation;    Cancer;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Vγ9Vδ2 T cells are non-canonical T cells that use their T cell receptor to detect phosphoantigens bound to the internal domain of the HMBPP receptor (butyrophilin 3/2A1 complex). This protocol describes the expansion and purification of human effector Vγ9Vδ2 T cells from human buffy coat and describes how to assess their activation by antigen-containing target cells. While specifically focused on cytokine production, this protocol can be readily adapted to evaluate other effector functions of activated Vγ9Vδ2 T cells.For complete details on the use and execution of this protocol, please refer to Hsiao et al. (2022) and Hsiao and Wiemer (2018). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

【 授权许可】

Unknown